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首页> 外文期刊>Journal of Clinical Microbiology >Detection and Characterization of Novel Rotavirus Strains in the United States
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Detection and Characterization of Novel Rotavirus Strains in the United States

机译：在美国检测和鉴定新型轮状病毒菌株

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We recently established a rotavirus strain surveillance system in the United States to monitor the prevalent G serotypes before and after the anticipated implementation of a vaccination program against rotavirus and to identify the emergence of uncommon strains. In this study, we examined 348 rotavirus strains obtained in 1996 to 1997 from children with diarrhea in 10 U.S. cities. Strains were characterized for P and G types, subgroups, and electropherotypes by using a combination of monoclonal antibody immunoassay, reverse transcription-PCR, and hybridization. The four strains most commonly found worldwide comprised 83% of the isolates (P[8]G1, 66.4%; P[4]G2, 8.3%; P[8]G3, 6.9%; P[8]G4, 1.4%), but 9.2% were unusual strains (P[6]G9, 5.5%; P[8]G9, 1.7%; P[6]G1, 1.4%; and P[4]G1 and P[8]G2, 0.3% each). Strains not typeable for P or G type accounted for 5.5% of the total, while 2.3% of the strains had more than one G type (mixed infections). All P[6]G9 strains tested had short electropherotypes and subgroup I specificity and were detected in 4 of 10 cities, while P[8]G9 strains had long electropherotypes and subgroup II VP6 antigens. Both sequence analysis of the VP7 open reading frame (about 94 to 95% amino acid identity with the VP7 gene of G9 prototype strain WI61) and binding to a G9-specific monoclonal antibody strongly suggest that U.S. G9 strains belong to serotype G9. The high detection rates of unusual rotaviruses with G9 (7.2%) or P[6] (6.9%) specificity in multiple U.S. cities suggest the emergence of new strains or inadequate diagnosis in the past. The epidemiologic importance of these strains remains to be determined.
机译：我们最近在美国建立了轮状病毒毒株监测系统，以在预期实施针对轮状病毒的疫苗接种计划之前和之后监测流行的G血清型，并识别罕见的毒株。在这项研究中，我们研究了1996年至1997年从美国10个城市的腹泻儿童那里获得的348种轮状病毒毒株。通过使用单克隆抗体免疫测定，逆转录-PCR和杂交的组合，对菌株进行P和G型，亚型和电泳型的鉴定。全球最常见的四种菌株占分离株的83％（P [8] G1，66.4％; P [4] G2，8.3％; P [8] G3，6.9％; P [8] G4，1.4％） ，但9.2％是异常菌株（P [6] G9，5.5％; P [8] G9，1.7％; P [6] G1，1.4％; P [4] G1和P [8] G2，0.3％每）。无法分型为P型或G型的菌株占总数的5.5％，而2.3％的菌株具有一种以上的G型（混合感染）。所有测试的P [6] G9菌株均具有短电型和I亚型特异性，并且在10个城市中有4个被检测到，而P [8] G9菌株具有长电型和II亚型VP6抗原。 VP7开放阅读框的序列分析（与G9原型菌株WI61的VP7基因约94至95％的氨基酸同一性）和与G9特异性单克隆抗体的结合都强烈表明美国G9菌株属于血清型G9。在美国多个城市中，具有G9（7.2％）或P [6]（6.9％）特异性的不寻常轮状病毒的检出率很高，这表明过去出现了新菌株或诊断不充分。这些菌株的流行病学重要性尚待确定。

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