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首页> 外文期刊>Journal of Clinical Microbiology >Serotypes of Escherichia coli that hybridized with DNA probes for genes encoding Shiga-like toxin I, Shiga-like toxin II, and serogroup O157 enterhemorrhagic E. coli fimbriae isolated from adults with diarrhea in Thailand.
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Serotypes of Escherichia coli that hybridized with DNA probes for genes encoding Shiga-like toxin I, Shiga-like toxin II, and serogroup O157 enterhemorrhagic E. coli fimbriae isolated from adults with diarrhea in Thailand.

机译：与DNA探针杂交的大肠杆菌血清型，这些基因编码了从泰国腹泻成年人中分离出的志贺样毒素I，志贺样毒素II和血清型O157肠出血性大肠杆菌菌毛的基因。

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Escherichia coli strains isolated from adults with diarrhea in Bangkok, Thailand, were examined for hybridization with DNA probes for genes that code for Shiga-like toxin (SLT)-I, SLT-II, and serogroup O157 enterhemorrhagic E. coli (EHEC) fimbriae. Seven isolates that hybridized with the SLT-I, SLT-II, and O157 EHEC fimbria probes and produced verocytotoxin (VT; group A) were isolated from two patients with diarrhea. Two strains that hybridized with only the SLT-II probe and the O157 EHEC fimbria probe and were VT+ (group B) were isolated from two patients with diarrhea, 7 strains that hybridized with only the SLT-II probe and were VT+ (group C) were isolated from two patients with diarrhea, and 26 strains that hybridized with only the O157 EHEC fimbria probe and were VT- (group D) were isolated from four patients with diarrhea. Seven strains in group A had serotypes O2:H1 (n = 1), O110:H19 (n = 1), and Ont:H8 (n = 5); 2 strains in group B were O112ab:H21 (n = 1) and O113:H21 (n = 1); 7 strains in group C were O6:H28 (n = 1), O22:H16 (n = 1), O52:H25 (n = 1), O112ab:H21 (n = 1), OR:H45 (n = 2), and OR:H11 (n = 1); and 26 strains in group D were O76:H7 (n = 18), O146:H3 (n = 2), O146:H10 (n = 1), O146:Hnt (n = 1), OR:H16 (n = 1), Ont:H2 (n = 1), Ont:H8 (n = 1) and Ont:H16 (n = 1). In Thailand, E. coli strains that hybridized with SLT-I, SLT-II, and O157 EHEC fimbria probes were of a variety of serotypes, none of which were O157:H7.
机译：检查了在泰国曼谷从腹泻成人中分离出的大肠杆菌菌株与DNA探针的杂交情况，以编码志贺样毒素（SLT）-I，SLT-II和血清型O157肠出血性大肠杆菌（EHEC）菌毛的基因。 。从两名腹泻患者中分离出了与SLT-1，SLTII和O157 EHEC菌毛探针杂交并产生病毒毒素的7种分离株（VT; A组）。从两名腹泻患者中分离出仅与SLT-II探针和O157 EHEC菌毛探针杂交并为VT +的两个菌株，B组为7个仅与SLT-II探针杂交且为VT +的菌株（C组）从两名腹泻患者中分离出了26株仅与O157 EHEC菌毛探针杂交且为VT-的菌株（D组），从四名腹泻患者中分离出。 A组中的7个菌株的血清型分别为O2：H1（n = 1），O110：H19（n = 1）和Ont：H8（n = 5）; B组2株为O112ab：H21（n = 1）和O113：H21（n = 1）; C组中的7个菌株为O6：H28（n = 1），O22：H16（n = 1），O52：H25（n = 1），O112ab：H21（n = 1）或OR：H45（n = 2） ，或OR：H11（n = 1）; D组中的26株是O76：H7（n = 18），O146：H3（n = 2），O146：H10（n = 1），O146：Hnt（n = 1）或OR：H16（n = 1） ），Ont：H2（n = 1），Ont：H8（n = 1）和Ont：H16（n = 1）。在泰国，与SLT-1，SLT-II和O157 EHEC菌毛探针杂交的大肠杆菌菌株具有多种血清型，但都不是O157：H7。

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