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首页> 外文期刊>Journal of Clinical Microbiology >Relative value of selective group A streptococcal agar incubated under different atmospheres.
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Relative value of selective group A streptococcal agar incubated under different atmospheres.

机译：在不同气氛下孵育的选择性A组链球菌琼脂的相对价值。

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摘要

A commercially available selective group A streptococcal agar (ssA) was evaluated for the recovery of group A streptococci (GAS) in comparison with recovery from simultaneous cultures on conventional sheep blood agar (SBA). Both sets of plates were incubated in air, 5% CO2, and anaerobically for 48 h, with a first reading taken at 24 h. A total of 402 (67.0%) GAS were isolated from the 600 specimens that were submitted. Recovery of GAS was significantly greater after 48 h of incubation than after 24 h of incubation for each medium-atmosphere combination. After 48 h of incubation, the sensitivities of GAS detection obtained by each culture technique were as follows: ssA-anaerobic atmosphere, 98.5%; SBA-anaerobic atmosphere, 89.5%; ssA-CO2 atmosphere, 88.0%; SBA-air, 86.5%; SBA-CO2 atmosphere, 82.0%; and ssA-air, 74.6%. There were no cultures positive in air or CO2 which were not positive anaerobically on either medium. The increased sensitivity of detecting positive GAS cultures when incubation was done in an ssA-anaerobic atmosphere for 48 h uncovered patients truly infected with the organisms.
机译：与常规羊血琼脂（SBA）上同时培养的回收率相比，评估了市售选择性A组链球菌琼脂（ssA）的回收率。将两组板在空气，5％CO2中孵育，并厌氧培养48小时，并于24小时进行首次读数。从提交的600个标本中总共分离出402个（67.0％）GAS。对于每种培养基-大气组合，孵育48小时后的GAS回收率均显着高于孵育24小时后。孵育48小时后，通过每种培养技术获得的GAS检测的灵敏度如下：ssA-厌氧气氛，98.5％; SBA-厌氧气氛，89.5％; ssA-CO2气氛，88.0％; SBA-空气，86.5％; SBA-CO2气氛，82.0％;和ssA-air，占74.6％。在空气或二氧化碳中没有阳性培养，在任何一种培养基上都没有厌氧阳性培养。在ssA-厌氧环境中孵育48小时后，检测到阳性GAS培养物的灵敏度提高了，但未发现真正感染该生物的患者。

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