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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of colistin and nalidixic acid in Todd-Hewitt broth for selective isolation of group B streptocci.
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Evaluation of colistin and nalidixic acid in Todd-Hewitt broth for selective isolation of group B streptocci.

机译:评估Todd-Hewitt肉汤中的大肠菌素和萘啶酸对B组链球菌的选择性分离。

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摘要

Todd-Hewitt broth (THB) containing colistin and nalidixic acid was compared with four other media with respect to efficacy of isolation of group B streptocci. It was effective as plain THB, THB with gentamicin and nalidixic acid and blood, and THB with colistin, nalidixic acid, and blood. THB with gentamicin and nalidixic acid, but without blood, was inhibitory to group B streptocci. The value of THB with colistin and nalidixic acid lies in its ability to successfully inhibit gram-negative organisms and still promote group B streptococcal growth without the addition of blood. This greatly reduces the time and expense of media preparation and permits early determination of bacterial growth, so that other means of rapidly identifying group B streptocci can be applied at the earliest possible time.
机译:就分离B组链球菌的功效而言,将含有大肠菌素和萘啶酸的Todd-Hewitt肉汤(THB)与其他四种培养基进行了比较。它与普通THB,庆大霉素和萘啶酸和血液中的THB以及粘菌素,萘啶酸和血液中的THB一样有效。含有庆大霉素和萘啶酸的THB对B组链球菌具有抑制作用,但无血。 THB与粘菌素和萘啶酸的价值在于其能够成功抑制革兰氏阴性菌并仍在不添加血液的情况下促进B组链球菌生长的能力。这大大减少了培养基制备的时间和费用,并允许及早确定细菌的生长,因此可以在最早的时间使用其他快速鉴定B组链球菌的方法。

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