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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of PASCO MIC-ID system for identifying gram-negative bacilli.
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Evaluation of PASCO MIC-ID system for identifying gram-negative bacilli.

机译:评估PASCO MIC-ID系统以鉴定革兰氏阴性杆菌。

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A production model of the semi-automated PASCO MIC-ID system (PASCO Laboratories, Wheat Ridge, Colo.) was evaluated with 122 groups or species of gram-negative bacilli, which included typical (499 cultures) and atypical (37 cultures) strains of fermenters and nonfermenters. The PASCO system identified 90.9% of 536 cultures accurately; these included 90.8% of 152 nonfermenters, 93.8% of 308 enteric fermenters, and 78.9% of 76 oxidase-positive fermenters. These results were obtained with the aid of serologic tests and a few additional biochemical tests, when recommended by the PASCO system. Of the 14 misidentified nonfermenters, 3 were Pseudomonas paucimobilis, 3 were Weeksella virosa (Centers for Disease Control group IIf), 2 were Xanthomonas (Pseudomonas) maltophilia, and 6 were randomly distributed among the other groups and species tested. The 19 enteric fermenters that were misidentified were randomly distributed among the groups and species tested. Of the 16 misidentified oxidase-positive fermenters, 4 were Pasteurella ureae, and 12 were randomly distributed among the other groups and species. The system identified the most commonly encountered organisms at a rate of 95% or better. The PASCO system is easy to inoculate and read. A slightly improved data base should remedy most of the identification problems.
机译:对半自动化的PASCO MIC-ID系统(PASCO实验室,科罗拉多州小麦里奇)的生产模型进行了评估,其中包括122组或革兰氏阴性菌种,其中包括典型菌株(499种)和非典型菌株(37种)。发酵罐和非发酵罐。 PASCO系统可准确识别536种培养物中的90.9%;其中包括152个非发酵罐中的90.8%,308个肠发酵罐中的93.8%和76个氧化酶阳性发酵罐中的78.9%。当PASCO系统推荐时,这些结果是通过血清学测试和一些其他生化测试获得的。在14个错误识别的非发酵菌中,有3个是假单胞假单胞菌,有3个是维氏周炎(疾病控制中心IIf组),有2个是黄单胞菌(假单胞菌)嗜麦芽炎,其余6个随机分布在其他组和测试物种中。被错误识别的19个肠道发酵罐随机分布在测试的组和物种中。在16个错误识别的氧化酶阳性发酵罐中,有4个是脲原巴斯德氏菌,在其他组和物种中随机分配了12个。该系统以95%或更高的比率识别出最常见的生物。 PASCO系统易于接种和阅读。稍有改进的数据库应该可以解决大多数识别问题。

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