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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of three dispersion procedures for quantitative recovery of cultivable species of subgingival spirochetes.
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Comparison of three dispersion procedures for quantitative recovery of cultivable species of subgingival spirochetes.

机译：比较三种分散程序定量回收龈下螺旋体可培养物种的方法。

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摘要

Spirochetes are usually the predominant organisms observed microscopically in subgingival plaques removed from tooth sites associated with periodontitis, but these organisms are rarely isolated by cultural means, presumably because the media do not support their growth and/or because these fragile organisms are disrupted by the various procedures used to disperse plaque samples. In the present investigation, three dispersal procedures, sonification, mechanical mixing, and homogenization, were compared for their ability to permit the isolation of Treponema denticola, Treponema vincentii, Treponema socranskii, and Treponema pectinovorum from plaque samples on media that support the growth of these species. Plaque samples in which the spirochetes averaged 50% of the microscopic count were chosen. The highest viable recoveries of spirochetes were observed when the plaques were dispersed with a Tekmar homogenizer, and the lowest occurred with sonification. The highest recoveries averaged only about 1% of the total cultivable counts, indicating either that the sought-after species were minor members of the flora or that the dispersal procedures were still too harsh. A total of 91% of the isolates were T. denticola, 5% were T. socranskii, and 4% were a heretofore-undescribed species.
机译：螺旋藻通常是从与牙周炎相关的牙齿部位去除的龈下斑块中微观观察到的主要生物，但是这些生物很少通过文化手段分离出来，大概是因为培养基不支持它们的生长和/或因为这些脆弱的生物被各种生物破坏了用于分散噬菌斑样品的程序。在本研究中，比较了三种分散程序，超声处理，机械混合和均质化的能力，以便能够从支持噬菌体的斑块样本中分离出密螺旋体，文氏螺旋体，松果螺旋体和果胶螺旋体的能力得以分离。种类。选择斑纹样品，其中螺旋体平均为显微计数的50％。用Tekmar匀浆器分散菌斑时，可观察到的螺旋体回收率最高，而超声处理则最低。最高的回收率平均仅占可耕种总数的1％，这表明，所追寻的物种是植物区系的次要成员，或者分散程序仍然过于苛刻。总共91％的分离物是T. denticola，5％是T. socranskii，4％是迄今未描述的物种。

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