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首页> 外文期刊>Journal of Clinical Microbiology >Genome fingerprinting of Pseudomonas aeruginosa indicates colonization of cystic fibrosis siblings with closely related strains.
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Genome fingerprinting of Pseudomonas aeruginosa indicates colonization of cystic fibrosis siblings with closely related strains.

机译：铜绿假单胞菌的基因组指纹图谱表明，囊性纤维化同胞的定殖与密切相关的菌株有关。

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The epidemiology of Pseudomonas aeruginosa infection at a cystic fibrosis (CF) center was monitored over a 3-year period. A total of 835 isolates from 72 unrelated patients and 22 siblings with CF were analyzed by genome fingerprinting and serotyping, bacteriophage typing, and pyocin typing. For genome fingerprinting, bacterial chromosomes were digested with one of the restriction endonucleases SpeI, DraI, XbaI, SspI, and NheI, which cut only rarely, and subsequently separated by field inversion gel electrophoresis. The physical genome analysis allowed us to classify P. aeruginosa strains in terms of DNA relatedness. Related strains differed by fewer than six DraI bands in the fingerprint, whereas unrelated strains differed by more than 20 DraI bands. All unrelated CF patients were colonized with different strains. The absence of a nosocomial spread of organisms at the CF center was attributed to the strict hygiene measures observed at the hospital. CF siblings were harboring either identical or closely related strains; transmission within the family is thought to be the most likely cause.
机译：在3年的时间内对铜绿假单胞菌在囊性纤维化（CF）中心的流行病学进行了监测。通过基因组指纹图谱和血清分型，噬菌体分型和幽门螺杆菌分型对来自72个无亲缘关系的患者和22个具有CF的兄弟姐妹的835个分离株进行了分析。对于基因组指纹图谱，细菌染色体用限制性内切酶SpeI，DraI，XbaI，SspI和NheI中的一种进行消化，这些酶很少切割，随后通过现场倒置凝胶电泳进行分离。物理基因组分析使我们能够根据DNA相关性对铜绿假单胞菌菌株进行分类。相关菌株在指纹中的差异少于六个DraI条带，而无关菌株之间的差异超过20条DraI区段。所有无关的CF患者均定植不同的菌株。 CF中心没有医院内细菌传播，这归因于医院严格的卫生措施。 CF兄弟姐妹携带相同或密切相关的菌株。家庭内部的传播被认为是最可能的原因。

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