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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of directigen RSV with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus.
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Comparison of directigen RSV with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus.

机译：Directigen RSV与病毒分离和直接免疫荧光的比较用于鉴定呼吸道合胞病毒。

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摘要

An enzyme immunoassay membrane test (Directigen RSV) for the detection of respiratory syncytial virus in clinical specimens was compared prospectively with isolation in cell culture and direct immunofluorescence (IF). A total of 315 nasopharyngeal wash specimens from pediatric patients were examined. Directigen RSV was 86.1% sensitive and 91.3% specific for specimens positive by isolation in cell culture and/or IF, with 88.6% agreement. The false-positive rate was 16%; 2 of 20 specimens giving false-positive reactions by Directigen RSV were true-positives by blocking assay. Twenty-seven specimens (8.5%) whose results were initially uninterpretable by Directigen RSV due to filtration difficulties were diluted and upon retesting produced acceptable results. Sixty-three viral isolates and/or IF identifications of virus antigens representing seven virus groups other than respiratory syncytial virus were also found; cross-reactions between Directigen RSV and other viruses were not observed. Directigen RSV will be useful as an immediate procedure and in facilities lacking a comprehensive virology laboratory.
机译：前瞻性比较了用于检测临床标本中呼吸道合胞病毒的酶免疫分析膜试验（Directigen RSV）与在细胞培养中分离和直接免疫荧光（IF）。总共检查了315例来自小儿患者的鼻咽冲洗标本。通过在细胞培养和/或IF中进行分离，Directigen RSV对阳性样本的敏感性为86.1％，特异性为91.3％，一致性为88.6％。假阳性率为16％; Directigen RSV产生假阳性反应的20个标本中有2个是通过封闭分析得出的真阳性。稀释了因过滤困难而最初无法由Directigen RSV解释的结果的27个样本（8.5％），经过重新测试后产生了可接受的结果。还发现了代表呼吸道合胞病毒以外的七个病毒组的六十三种病毒分离株和/或中频鉴定。没有观察到Directigen RSV与其他病毒之间的交叉反应。 Directigen RSV可作为即时程序，也可用于缺乏综合病毒学实验室的设施。

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