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首页> 外文期刊>Journal of Clinical Microbiology >Rapid focus reduction neutralization test of influenza A and B viruses in microtiter system.
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Rapid focus reduction neutralization test of influenza A and B viruses in microtiter system.

机译：微量滴定系统中甲型和乙型流感病毒的快速聚焦减少中和测试。

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摘要

A rapid neutralization test for influenza A and B viruses was developed. In this method, a 96-well tissue culture plate was used for the preparation of cell monolayers and the peroxidase-antiperoxidase staining technique was used for the visualization of foci infected with these viruses. In the presence of trypsin and tragacanth gum, clear foci developed 1 day after infection. A linear relationship between virus dilutions and numbers of foci was observed. When neutralizing antibodies in some test sera were assayed, a good correlation was observed between the titers obtained by the focus method and those obtained by the ordinary plaque method. In addition, many serum specimens were investigated by the neutralization test, and it was demonstrated that the test is useful for serological studies of influenza.
机译：开发了针对甲型和乙型流感病毒的快速中和测试。在这种方法中，使用96孔组织培养板制备细胞单层，并使用过氧化物酶-抗过氧化物酶染色技术来可视化感染这些病毒的病灶。在存在胰蛋白酶和黄aga胶的情况下，感染后1天会形成清晰的病灶。观察到病毒稀释度与病灶数之间存在线性关系。在某些测试血清中测定中和抗体时，观察到通过聚焦法获得的效价与通过普通噬斑法获得的效价之间具有良好的相关性。此外，通过中和试验对许多血清标本进行了研究，结果表明该试验对流感的血清学研究很有用。

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