...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Human serum antibody response to the presence of Aeromonas spp. in the intestinal tract.
【24h】

Human serum antibody response to the presence of Aeromonas spp. in the intestinal tract.

机译：人血清抗体对气单胞菌属的存在的反应。在肠道。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A bacterial agglutination assay, a toxin-neutralizing assay, and an enzyme-linked immunosorbent assay (ELISA) were used to compare antibodies against intestinal Aeromonas strains in serum samples from healthy carriers (n = 6), from patients with acute (n = 15) or chronic (n = 8) gastroenteritis, from patients with gastroenteritis caused by other enteropathogenic bacteria (n = 3), and from healthy blood donors (n = 50). Evaluation of the bacterial agglutination assay showed that it was not very useful. The sensitivity of the ELISA in patients with acute or chronic aeromonas-associated diarrhea was 30% (7 of 23 patients were positive), whereas the specificity was 74% (13 of 50 healthy donors were positive). Positive results in the ELISA correlated with immunoglobulin M and immunoglobulin G responses to lipopolysaccharides of homologous Aeromonas strains, as determined by gel immunoradioassay and Western immunoblot analysis. The sera showed cross-reactions with heterologous Aeromonas strains and with Escherichia coli strains. The toxin-neutralizing assay was positive in 5 of 11 patients who had developed acute severe diarrhea associated with cytotoxin-producing Aeromonas strains (46% sensitivity), whereas only 3 of 50 healthy donors had low serum titers of cytotoxin-neutralizing antibodies (94% specificity). All five patients were over 60 years of age. Cytotoxin-neutralizing activity was not observed in the sera of other groups of patients with aeromonads in their feces. We concluded that the three different serologic assays were not consonant with one another and that only the toxin-neutralizing assay distinguished patients with acute diarrhea from other groups of patients.
机译：细菌凝集测定，毒素中和测定和酶联免疫吸附测定（ELISA）用于比较健康携带者（n = 6），急性患者（n = 15）的血清样品中针对肠道气单胞菌菌株的抗体）或慢性（n = 8）肠胃炎，其他肠致病细菌引起的肠胃炎患者（n = 3）和健康献血者（n = 50）。细菌凝集测定的评估表明它不是很有用。 ELISA对患有急性或慢性气溶胶相关性腹泻的患者的敏感性为30％（23名患者中有7名阳性），而特异性为74％（50名健康供体中有13名阳性）。 ELISA中的阳性结果与免疫球蛋白M和免疫球蛋白G对同源气单胞菌菌株的脂多糖的反应有关，这通过凝胶免疫放射分析法和Western免疫印迹分析确定。血清显示与异源气单胞菌菌株和大肠杆菌菌株的交叉反应。毒素中和试验在11例发生急性严重腹泻的患者中有5例阳性，这些患者与产生细胞毒素的气单胞菌菌株有关（敏感性为46％），而50名健康供体中只有3名血清中和细胞毒素的抗体的滴度较低（94％特异性）。所有五名患者均超过60岁。在其他粪便中有气单胞菌的患者的血清中未观察到细胞毒素中和活性。我们得出的结论是，三种不同的血清学检测方法彼此不一致，只有中和毒素的检测方法才能将急性腹泻患者与其他患者组区分开。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号