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首页> 外文期刊>Journal of Clinical Microbiology >Influence of endocervical specimen adequacy on PCR and direct fluorescent-antibody staining for detection of Chlamydia trachomatis infections.
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Influence of endocervical specimen adequacy on PCR and direct fluorescent-antibody staining for detection of Chlamydia trachomatis infections.

机译：子宫颈内标本充分性对PCR和直接荧光抗体染色检测沙眼衣原体感染的影响。

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The cellular quality of the endocervical swab specimen used for the detection of Chlamydia trachomatis may dramatically impact the sensitivity of the diagnostic assay used. An evaluation of the adequacy of 319 endocervical swab specimens from women attending two inner-city sexually transmitted disease and family planning clinics, as well as five high school-based family planning clinics, was performed, and the resulting data were compared with the diagnostic results obtained by both Amplicor PCR and Microtrak direct fluorescent-antibody (DFA) staining. The swab from each patient was rolled across the open circular area of a DFA slide and then used to inoculate a transport tube for PCR (Roche), after which the swab was discarded. The slides were stained and examined by epifluorescence microscopy for the presence of C. trachomatis elementary bodies and for the presence and number of cell types to determine specimen adequacy. Cellular adequacy for a cervical swab specimen was defined as the presence of one or more columnar epithelial or metaplastic epithelial cells or the presence of more than 100 erythrocytes per high-power microscopic field. Of the 319 specimens read by DFA, 204 (63.9%) were determined to be adequate. There were 34 (10.7%) positive specimens by DFA and/or PCR. Twenty-nine (9.1%) specimens were positive by PCR, 20 (6.3%) specimens were DFA positive, and 15 (4.7%) were concordantly positive by both tests. The prevalence of chlamydia among adequate specimens was 14.2% (29/204), compared to 4.3% (5/115) for inadequate specimens (P < 0.0001). Variations in specimen quality and the sensitivity of the diagnostic assay used have a significant impact on determining the prevalence of C. trachomatis in a population.
机译：用于检测沙眼衣原体的子宫颈拭子样本的细胞质量可能会极大地影响所用诊断测定的灵敏度。评估了来自在两个市内性传播疾病和计划生育诊所以及五个高中的计划生育诊所就诊的妇女的319例宫颈拭子样本的充分性，并将所得数据与诊断结果进行了比较通过Amplicor PCR和Microtrak直接荧光抗体（DFA）染色获得。将每位患者的拭子在DFA载玻片的圆形开口区域滚动，然后用于接种用于PCR（Roche）的运输管，然后将拭子弃去。将载玻片染色并通过落射荧光显微镜检查，以检测沙眼衣原体基本体的存在以及细胞类型的存在和数量，以确定样品的适当性。宫颈拭子标本的细胞充分性定义为每个高倍镜视野中存在一种或多种柱状上皮或化生上皮细胞，或存在100多个红细胞。通过DFA读取的319个样本中，有204个（63.9％）被确定为足够。通过DFA和/或PCR检出34份（10.7％）阳性标本。通过PCR检出29例（9.1％）阳性，DFA阳性20例（6.3％），两次测试均15例（4.7％）一致。适当标本中衣原体的患病率为14.2％（29/204），而不适当标本中披衣菌的患病率为4.3％（5/115）（P <0.0001）。标本质量的变化和所用诊断方法的敏感性对确定人群中沙眼衣原体的患病率具有重大影响。

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