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首页> 外文期刊>Journal of Clinical Microbiology >Human Papillomavirus (HPV) DNA Copy Number Is Dependent on Grade of Cervical Disease and HPV Type
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Human Papillomavirus (HPV) DNA Copy Number Is Dependent on Grade of Cervical Disease and HPV Type

机译：人乳头瘤病毒（HPV）DNA拷贝数取决于宫颈疾病的等级和HPV类型

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The association between human papillomavirus (HPV) DNA copy number and cervical disease was investigated. Viral DNA copy number for the most common high-risk HPV types in cervical cancer (types 16, 18, 31, and 45) was determined in cervical cytobrush specimens from 149 women with high-grade cervical intraepithelial neoplasias (CIN II-CIN III), 176 with low-grade CIN (CIN I), and 270 with normal cytology. Quantitative, PCR-based fluorescent assays for each of the HPV genotypes and for the β-globin gene were used. The amount of cellular DNA increased significantly with increasing disease; thus, HPV was expressed as copies per microgram of cellular DNA. The assay had a dynamic range of >107, allowing documentation for the first time of the wide range of HPV copy numbers seen in clinical specimens. Median HPV DNA copy number varied by more than 104 among the viral types. HPV16 was present in the highest copy number; over 55% of HPV16-positive samples contained more than 108 copies/μg. Median copy number for HPV16 showed dramatic increases with increasing epithelial abnormality, an effect not seen with the other HPV types. HPV16 increased from a median of 2.2 × 107 in patients with normal cytology, to 4.1 × 107 in CIN I patients, to 1.3 × 109 copies/μg in CIN II-III patients. Even when stratified by cervical disease and viral type, the range of viral DNA copies per microgram of cellular DNA was quite large, precluding setting a clinically significant cutoff value for “high” copy numbers predictive of disease. This study suggests that the clinical usefulness of HPV quantitation requires reassessment and is assay dependent.
机译：研究了人类乳头瘤病毒（HPV）DNA拷贝数与宫颈疾病之间的关系。在149例高度宫颈上皮内瘤变（CIN II-CIN III）妇女的宫颈细胞刷标本中确定了宫颈癌中最常见的高危HPV类型（16、18、31和45型）的病毒DNA拷贝数，具有低级CIN（CIN I）的176和具有正常细胞学特征的270。对每种HPV基因型和β-珠蛋白基因使用基于PCR的定量荧光分析。随着疾病的增加，细胞DNA的数量显着增加。因此，HPV表示为每微克细胞DNA的拷贝数。该测定法的动态范围> 10 7 ，从而首次记录了在临床标本中发现的多种HPV拷贝数。在病毒类型之间，HPV DNA的中位拷贝数差异超过10 4 。 HPV16的拷贝数最高;超过55％的HPV16阳性样品含有超过10 8 个/μg。 HPV16的中位拷贝数随着上皮异常的增加而显着增加，而其他HPV类型则没有这种作用。细胞学检查正常的患者中，HPV16的中位数从2.2×10 7 增加到CIN I患者的4.1×10 7 ，增加到1.3×10 9 拷贝/μg在CIN II-III患者中。即使按宫颈疾病和病毒类型分层，每微克细胞DNA的病毒DNA拷贝范围也相当大，这排除了为预测疾病的“高”拷贝数设定临床上有意义的临界值。这项研究表明，HPV定量的临床有用性需要重新评估，并且取决于测定。

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