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首页> 外文期刊>Journal of Clinical Microbiology >Multilocus Sequence Typing for Characterization of Methicillin-Resistant and Methicillin-Susceptible Clones ofStaphylococcus aureus
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Multilocus Sequence Typing for Characterization of Methicillin-Resistant and Methicillin-Susceptible Clones ofStaphylococcus aureus

机译：多基因座序列分型用于鉴定金黄色葡萄球菌的耐甲氧西林和耐甲氧西林的克隆

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A multilocus sequence typing (MLST) scheme has been developed forStaphylococcus aureus. The sequences of internal fragments of seven housekeeping genes were obtained for 155 S. aureusisolates from patients with community-acquired and hospital-acquired invasive disease in the Oxford, United Kingdom, area. Fifty-three different allelic profiles were identified, and 17 of these were represented by at least two isolates. The MLST scheme was highly discriminatory and was validated by showing that pairs of isolates with the same allelic profile produced very similar SmaI restriction fragment patterns by pulsed-field gel electrophoresis. All 22 isolates with the most prevalent allelic profile were methicillin-resistant S. aureus (MRSA) isolates and had allelic profiles identical to that of a reference strain of the epidemic MRSA clone 16 (EMRSA-16). Four MRSA isolates that were identical in allelic profile to the other major epidemic MRSA clone prevalent in British hospitals (clone EMRSA-15) were also identified. The majority of isolates (81%) were methicillin-susceptible S. aureus (MSSA) isolates, and seven MSSA clones included five or more isolates. Three of the MSSA clones included at least five isolates from patients with community-acquired invasive disease and may represent virulent clones with an increased ability to cause disease in otherwise healthy individuals. The most prevalent MSSA clone (17 isolates) was very closely related to EMRSA-16, and the success of the latter clone at causing disease in hospitals may be due to its emergence from a virulent MSSA clone that was already a major cause of invasive disease in both the community and hospital settings. MLST provides an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the Internet.
机译：针对金黄色葡萄球菌开发了一种多基因座序列分型（MLST）方案。获得了155个 S的七个管家基因的内部片段的序列。金黄色葡萄球菌是从英国牛津地区的社区获得性和医院获得性浸润性疾病患者中分离出来的。鉴定出53种不同的等位基因谱，其中17种由至少两个分离株代表。 MLST方案具有高度区分性，并且通过显示具有相同等位基因特征的分离株对通过脉冲场凝胶电泳产生非常相似的 Sma I限制性片段模式得到了验证。具有最普遍的等位基因特征的所有22个分离株均为耐甲氧西林的 S。金黄色葡萄球菌（MRSA）分离株，并具有与流行性MRSA克隆16（EMRSA-16）参考菌株相同的等位基因特征。还鉴定了四个等位基因特征与英国医院中流行的其他主要流行性MRSA克隆（克隆EMRSA-15）相同的MRSA分离株。多数分离株（81％）是对甲氧西林敏感的 S。金黄色葡萄球菌（MSSA）分离株，七个MSSA克隆包括五个或更多分离株。 MSSA克隆中的三个包括至少五个来自社区获得性浸润性疾病患者的分离株，并且可能代表强力克隆，在其他情况下健康的个体中引起疾病​​的能力增强。最普遍的MSSA克隆（17个分离株）与EMRSA-16密切相关，后者的克隆在医院引起疾病的成功可能归因于它已经从强毒的MSSA克隆中出现，而该MSSA克隆已经是侵袭性疾病的主要原因在社区和医院环境中。 MLST提供了一种明确的方法，用于将MRSA和MSSA分离物分配给已知克隆，或者通过Internet将它们分配为新克隆。

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