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首页> 外文期刊>Journal of Clinical Microbiology >Rapid Detection of Contagious Caprine Pleuropneumonia Using aMycoplasma capricolum subsp. capripneumoniaeCapsular Polysaccharide-Specific Antigen Detection Latex Agglutination Test
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Rapid Detection of Contagious Caprine Pleuropneumonia Using aMycoplasma capricolum subsp. capripneumoniaeCapsular Polysaccharide-Specific Antigen Detection Latex Agglutination Test

机译：快速检测传染性山羊胸膜肺炎使用支原体亚种。肺炎克雷伯菌荚膜多糖特异性抗原检测乳胶凝集试验

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Latex microspheres (diameter, 8 μm) were coated with anti-Mycoplasma capricolum subsp.capripneumoniae polyclonal immunoglobulin G (IgG) antiserum (anti-F38 biotype). The coated microspheres, when used in a latex agglutination test (LAT), detected M. capricolumsubsp. capripneumoniae antigen in the serum of goats with contagious caprine pleuropneumoniae (CCPP). Beads also agglutinated strongly in the presence of purified M. capricolumsubsp. capripneumoniae capsular polysaccharide (CPS). Preabsorption of CPS-specific antibodies prior to coating of the beads removed agglutinating activity in the presence of M. capricolum subsp. capripneumoniae, strongly suggesting that CPS is the likely soluble antigen recognized by the test. In addition, the specificity of the LAT exactly mirrored that of an M. capricolum subsp. capripneumoniaeCPS-specific monoclonal antibody (WM25): of the 8 other mycoplasma species tested, agglutination was observed only with bovine serogroup 7. The LAT detected all 11 strains of M. capricolumsubsp. capripneumoniae examined in this study, with a sensitivity level of 2 ng of CPS, or the equivalent of 1.7 × 104 CFU, in a reaction volume of 0.03 ml of serum. With field sera from goats with CCPP, the results of the LAT exhibited a 67% correlation with the results of the currently used complement fixation test (CFT), with the main discrepancy in diagnosis resulting from the increased sensitivity of the LAT compared to that of CFT. This antigen-detection LAT should prove particularly useful in identifying animals in the earliest stages of CCPP and combines sensitivity and low cost with ease of application in the field, without the need for any specialist training or equipment.
机译：乳胶微球（直径为8μm）涂有抗 Capcoolum 亚种 neumcaponianeumoniae 多克隆免疫球蛋白G（IgG）抗血清（抗F38生物型）。当用于乳胶凝集试验（LAT）时，涂覆的微球检测到 M。随心所欲 subsp。传染性山羊胸膜肺炎球菌（CCPP）山羊血清中的 capripneumoniae 抗原。在纯化的 M的存在下，珠子也强烈凝集。随心所欲 subsp。 capripneumoniae 荚膜多糖（CPS）。在 M的存在下，对CPS特异性抗体的预吸收在包被珠子之前消除了凝集活性。随心所欲的子空间 capripneumoniae ，强烈表明CPS是该测试识别的可能可溶性抗原。此外，LAT的特异性与 M的特异性完全相同。随心所欲的子空间 capripneumoniae CPS特异性单克隆抗体（WM25）：在其他8种支原体中，只有牛血清群7才观察到凝集。LAT检测到所有11种 M菌株。随心所欲 subsp。在这项研究中检测的 capripneumoniae ，在0.03 ml血清反应体积中的敏感度为2 ng CPS，或相当于1.7×10 4 CFU。对于来自CCPP山羊的野外血清，LAT的结果与当前使用的补体固定试验（CFT）的结果显示67％的相关性，主要的诊断差异是由于LAT的敏感性比其他方法高。 CFT。这种抗原检测LAT在CCPP最早阶段的动物识别中应被证明特别有用，并且结合了灵敏度和低成本以及易于现场应用的优势，而无需任何专业培训或设备。

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