Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with OtherRalstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)

Didier Moissenet; Philippe Bidet; Antoine Garbarg-Chenon; Guillaume Arlet; Hoang Vu-Thien

首页> 外文期刊>Journal of Clinical Microbiology >Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with OtherRalstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)

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Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with OtherRalstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)

机译：Ralstonia paucula（以前是CDC Group IV c-2）：基于PCR的方法无法成功进行菌株分化，rRNA操纵子的16S-23S间隔子研究以及与其他Ralstonia物种的比较（R. eutropha，R。pickettii，R。gilardii和青枯菌（R. solanacearum）

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Ralstonia paucula (formerly CDC group IV c-2) can cause serious human infections. Confronted in 1995 with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, threeR. solanacearum strains, and eight R. gilardiistrains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described forR. pickettii and R. solanacearum.

机译： Ralstonia paucula （以前是CDC IV c-2组）可引起严重的人类感染。面对1995年的5例医院菌血症，我们发现脉冲场凝胶电泳无法区分分离株，而随机扩增多态性DNA分析的辨别能力很差。在这项研究中，我们对间隔区16S-23S核糖体DNA（rDNA）进行了PCR核糖体分型和PCR限制性片段长度多态性分析。两种方法都无法区分 R。 paucula 分离株。属于其他 Ralstonia 物种的18个菌株（1个 R。eutropha 菌株，6个 R.ettiettii 菌株，3个 R。solanacearum 还通过PCR核糖体分型法检测了8株 R。gilardii 菌株，但未能区分这4种。 em的16S-23S rDNA基因间隔子。 paucula 包含tRNA Ile 和tRNA Ala 基因，这些基因与针对 R的基因相同。 pickettii 和 R。茄科。 展开▼

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《Journal of Clinical Microbiology》 |2001年第1期|共4页

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Didier Moissenet; Philippe Bidet; Antoine Garbarg-Chenon; Guillaume Arlet; Hoang Vu-Thien;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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1. PM 7/21 (2) Ralstonia solanacearum, R. pseudosolanacearum and R. syzygii (Ralstonia solanacearum species complex) [J] . Bulletin OEPP: = EPPO Bulletin . 2018,第1期

机译：PM 7/21（2）Ralstonia solanacearum，R.Pseudosolanacearum和R. syzygii（Ralstonia solanacearum物种复合物）

2. Infection by Ralstonia Species in Cystic Fibrosis Patients: Identification of R. pickettii and R. mannitolilytica by Polymerase Chain Reaction [J] . T. Coenye, Peter Vandamme, John JLiPuma Emerging Infectious Diseases . 2002,第7期

机译：囊性纤维化患者中的罗尔斯通氏菌属感染：通过聚合酶链反应鉴定p。pickettii和r。mannitolilytica

3. Complete Genome Sequences of the Plant Pathogens Ralstonia solanacearum Type Strain K60 and R. solanacearum Race 3 Biovar 2 Strain UW551 [J] . Madeline M. Hayes, April M. MacIntyre, Caitilyn Allen Genome Announcements . 2017,第40期

机译：植物病原体Ralstonia solanacearum型菌株K60和R. solanacearum第3种Biovar 2菌株UW551的完整基因组序列。

4. Note: Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods Study of the 16S-23S Spacer of the rRNA Operon and Comparison with Other Ralstonia Species (R. eutropha R. pickettii R. gilardii and R. solanacearum) [O] . Didier Moissenet, Philippe Bidet, Antoine Garbarg-Chenon, 2001

机译：注意：Ralstonia paucula（以前是CDC Group IV c-2）：基于PCR的方法未能成功进行菌株分化rRNA操纵子的16S-23S间隔子研究以及与其他Ralstonia物种（R. eutrophaR。pickettii R. gilardii和R. solanacearum）

5. Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum) [O] . Moissenet, Didier, Bidet, Philippe, Garbarg-Chenon, Antoine, 2001

机译：Ralstonia paucula（以前是CDC Group IV c-2）：基于PCR的方法无法成功进行菌株分化，rRNA操纵子的16S-23S间隔子研究以及与其他Ralstonia物种的比较（R. eutropha，R. pickettii，R.吉拉第氏菌和青枯菌）

1. mutant strains of the species raoultella planticola, an industrially viable method for biotechnological transformation of glycerol to 2,3-butanediol, and method of obtaining mutant strains of the species r. planticola [P] . 外国专利： BR112015001094A2 . 2017-06-27

机译：种植物拉乌尔氏菌（Raoultella planticola）的突变株，将甘油生物技术转化为2，3-丁二醇的工业上可行的方法以及获得r。种的突变株的方法。植物

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Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with OtherRalstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)

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