Rapid Detection of Mutations in the 23S rRNA Gene of Helicobacter pylori That Confers Resistance to Clarithromycin Treatment to the Bacterium

Masayuki Matsumura; Yoko Hikiba; Keiji Ogura; Goichi Togo; Izumi Tsukuda; Kenji Ushikawa

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Rapid Detection of Mutations in the 23S rRNA Gene of Helicobacter pylori That Confers Resistance to Clarithromycin Treatment to the Bacterium

机译：幽门螺杆菌23S rRNA基因突变的快速检测，该突变赋予对细菌的克拉霉素治疗耐药性

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We developed a new method capable of detecting point mutations in the 23S rRNA gene of Helicobacter pylori using a LightCycler. Our method can detect a mutation in this gene in less than 1 h and can process many samples at once, thereby contributing to the selection of patients suitable for clarithromycin-based therapy.

机译：我们开发了一种新的方法，该方法可以使用LightCycler检测幽门螺杆菌（Helicobacter pylori）的23S rRNA基因中的点突变。我们的方法可以在不到1小时的时间内检测到该基因的突变，并且可以一次处理多个样本，从而有助于选择适合基于克拉霉素的疗法的患者。

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《Journal of Clinical Microbiology》 |2001年第2期|共5页
作者
Masayuki Matsumura; Yoko Hikiba; Keiji Ogura; Goichi Togo; Izumi Tsukuda; Kenji Ushikawa;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. PCR-RFLP detection of point mutations A2143G and A2142G in 23S rRNA gene conferring resistance to clarithromycin in Helicobacter pylori strains [J] . Acta biochimica Polonica . 2014,第2期

机译：PCR-RFLP检测23S rRNA基因中点突变A2143G和A2142G赋予幽门螺杆菌菌株对克拉霉素的抗性
2. Mutations in 23S rRNA in Helicobacter pylori conferring resistance to erythromycin do not always confer resistance to clarithromycin. [J] . Garcia Arata-MI, Baquero F, de Rafael-L, Antimicrobial agents and chemotherapy. . 1999,第2期

机译：幽门螺杆菌中的23S rRNA突变赋予对红霉素的抗性并不总是赋予对克拉霉素的抗性。
3. A novel RT-PCR for the detection of Helicobacter pylori and identification of clarithromycin resistance mediated by mutations in the 23S rRNA gene [J] . Redondo Javier Jareno, Keller Peter M., Zbinden Reinhard, Diagnostic microbiology and infectious disease . 2018,第1期

机译：一种用于检测幽门螺杆菌幽门螺杆菌的新型RT-PCR和23s rRNA基因突变介导的克拉霉素抗性的鉴定
4. Construction of nonlinear fractional order model for clarithromycin against helicobacter pylori and compared with integer order model [C] . Wenchao Wang, Yan Li, Yundong Sun Chinese Automation Congress . 2017

机译：克拉霉素对幽门螺杆菌的非线性分数阶模型的建立及与整数阶模型的比较
5. Cholesterol-dependent and HefC-mediated mechanisms of bile salt resistance in Helicobacter pylori. [D] . Trainor, Elizabeth Ashley. 2012

机译：幽门螺杆菌的胆固醇依赖性和HefC介导的胆盐抗性机制。
6. Rapid Detection of Mutations in the 23S rRNA Gene of Helicobacter pylori That Confers Resistance to Clarithromycin Treatment to the Bacterium [O] . Masayuki Matsumura, Yoko Hikiba, Keiji Ogura, 2001

机译：幽门螺杆菌23S rRNA基因突变的快速检测可赋予对细菌的克拉霉素抗药性
7. Rapid Detection of Mutations in the 23S rRNA Gene of Helicobacter pylori That Confers Resistance to Clarithromycin Treatment to the Bacterium [O] . Matsumura, Masayuki, Hikiba, Yoko, Ogura, Keiji, 2001

机译：幽门螺杆菌23S rRNA基因突变的快速检测，该突变赋予对细菌的克拉霉素治疗耐药性

Rapid Detection of Mutations in the 23S rRNA Gene of Helicobacter pylori That Confers Resistance to Clarithromycin Treatment to the Bacterium

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