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首页> 外文期刊>Journal of Clinical Microbiology >Rapid screening for bacteriuria by light scatter photometry (Autobac): a collaborative study.
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Rapid screening for bacteriuria by light scatter photometry (Autobac): a collaborative study.

机译：通过光散射光度法（Autobac）快速筛选细菌尿症：一项合作研究。

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A total of 2,720 urine specimens from three laboratories were evaluated by Autobac (Pfizer Diagnostics) and were compared with simultaneous colony counts for evidence of bacteriuria. Of 599 specimens with a colony count of greater than or equal to 10(5) colony-forming units per ml, 93.8% were detected within 6 h. This detection rate increased to 97% of 447 positive urine specimens when only specimens from patients not on antimicrobials were evaluated. The majority (77.9%) of positive specimens were detected as early as 3 h. Those specimens with greater than or equal to 10(5) colony-forming units per ml, which were negative by Autobac at 6 h, included organisms which are frequently considered to be contaminants (diphtheroids, lactobacilli, alpha and gamma streptococci, yeasts, and Staphylococcus epidermidis), or were from patients who were being treated with antimicrobial agents. Of 2,121 urine specimens with colony counts of less than 10(5), 98.1% were correctly determined to be negative by Autobac at 3 h. This percentage decreased to 86.0 at 6 h. The majority of these false-positive specimens were those with colony counts of 10(4) to 10(5) colony-forming units per ml. There appeared to be no major difference in results from the three laboratories or among the four lots of broth used in this study.
机译：Autobac（Pfizer Diagnostics）对来自三个实验室的总计2,720个尿液标本进行了评估，并将其与同时菌落计数进行比较，以发现有细菌尿的迹象。在599个样本中，每毫升中菌落计数大于或等于10（5）个菌落形成单位，在6小时内检测到93.8％。当仅评估未使用抗生素的患者的尿样时，该检出率增加到447个尿样阳性的97％。早在3 h即可检测到大部分阳性样本（77.9％）。那些标本每毫升大于或等于10（5）菌落形成单位的样本在6小时内被Autobac呈阴性，其中包括经常被视为污染物的生物（双环类，乳酸杆菌，α和γ链球菌，酵母菌和表皮葡萄球菌），或来自接受抗微生物剂治疗的患者。在3小时后，Autobac正确地确定了2,121个菌落计数低于10（5）的尿液样本中98.1％为阴性。该百分比在6小时时降至86.0。这些假阳性样本大多数是菌落计数为每毫升10（4）至10（5）的菌落形成单位。三个实验室或本研究使用的四个批次的肉汤的结果似乎没有重大差异。

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