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首页> 外文期刊>Journal of Clinical Microbiology >Comparative laboratory evaluation of three antigen detection methods for diagnosis of Haemophilus influenzae type b disease.
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Comparative laboratory evaluation of three antigen detection methods for diagnosis of Haemophilus influenzae type b disease.

机译：三种抗原检测方法在诊断b型流感嗜血杆菌中的对比实验室评估。

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Cerebrospinal fluid, urine, serum, and other body fluid specimens from pediatric patients with systemic disease were tested with Bactigen latex agglutination (555 specimens), Phadebact coagglutination (319 specimens), and counterimmunoelectrophoresis (335 specimens) for the presence of Haemophilus influenzae type b antigen. All three methods showed good sensitivity for detecting antigen in the cerebrospinal fluid of patients with culture-positive meningitis (greater than or equal to 86% sensitivity). However, coagglutination and counterimmunoelectrophoresis were much less sensitive (less than or equal to 40%) than latex agglutination (96%) for detecting antigen in other body fluid specimens in culture-positive, nonmeningeal H. influenzae disease. Bactigen latex agglutination was also more sensitive than the other procedures for detecting antigen in specimens from patients with culture-negative, presumed H. influenzae disease. Comparative testing of fluids spiked with known quantities of purified H. influenzae b polyribosephosphate capsular polysaccharide revealed an apparent 100-fold greater sensitivity with Bactigen as compared with the other two methods. Although all three methods showed good specificity (greater than 98%), both agglutination methods gave a few false-positive results. In a clinical setting where both meningeal and nonmeningeal H. influenzae b disease are encountered frequently, Bactigen latex agglutination appears to be superior to coagglutination and counterimmunoelectrophoresis for detecting antigen in body fluids.
机译：细菌原胶凝集（555标本），Phadebact凝集（319标本）和抗免疫电泳（335标本）检测了b型流感嗜血杆菌的存在，对患有系统疾病的小儿患者的脑脊液，尿液，血清和其他体液标本进行了检测。抗原。这三种方法对培养阳性脑膜炎患者的脑脊液中抗原的检测均显示出良好的灵敏度（灵敏度大于或等于86％）。但是，对于在培养阳性的非脑膜炎性流感嗜血杆菌病中检测其他体液标本中的抗原，凝集和反免疫电泳的敏感性（小于或等于40％）不及乳胶凝集（96％）。与其他方法相比，细菌原胶乳凝集也比其他方法更敏感，可用于检测培养阴性，推测为流感嗜血杆菌病患者的标本中的抗原。对掺有已知量的纯化的流感嗜血杆菌b聚核糖磷酸荚膜多糖的液体进行的对比测试显示，与其他两种方法相比，细菌原的敏感性明显提高了100倍。尽管这三种方法均显示出良好的特异性（大于98％），但两种凝集方法均给出了一些假阳性结果。在经常遇到脑膜和非膜性乙型流感嗜血杆菌疾病的临床环境中，细菌胶乳凝集似乎优于凝集和反免疫电泳来检测体液中的抗原。

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