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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of a blood-free, charcoal-based, selective medium for the isolation of Campylobacter organisms from feces.
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Evaluation of a blood-free, charcoal-based, selective medium for the isolation of Campylobacter organisms from feces.

机译：评价用于从粪便中分离弯曲杆菌的无血，基于木炭的选择性培养基。

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A blood-free, charcoal-based selective medium (CSM) consisting of a Columbia agar base, activated charcoal (4 g/liter), hematin (0.032 g/liter), sodium pyruvate (0.1 g/liter), cefoperazone (32 mg/liter), vancomycin (20 mg/liter), and cycloheximide (100 mg/liter) supported the growth of Campylobacter jejuni and C. coli with colony counts equivalent to those obtained on antibiotic-free horse blood agar. CSM was compared to Skirrow medium (SKM) for the recovery of C. jejuni and C. coli from stools of patients with diarrhea, the media being incubated for 2 days under reduced oxygen tension at 43 degrees C. These campylobacters were isolated from 35 (2.9%) of 1,227 stools tested (29 on both media, 5 on CSM alone, and one on SKM alone). Whenever C. jejuni and C. coli were recovered, growth was pure on 29 CSM cultures (85%), but on only 11 SKM cultures (37%). Complete suppression of "contaminating" flora occurred in 704 CSM cultures (57%) compared with 426 SKM cultures (35%). CSM more effectively suppressed contaminating pseudomonads, gram-positive organisms, and yeasts than did SKM; both media failed to suppress members of the family Enterobacteriaceae in about a quarter of the samples. Studies on 20 representative Enterobacteriaceae contaminants showed that susceptibility to cefoperazone and growth on CSM were markedly dependent on inoculum size; 12 strains were inhibited by cefoperazone (32 mg/liter) at inoculum sizes of 5 X 10(2) and 5 X 10(4) but not 5 X 10(6) organisms, indicating that the frequency of contaminants on CSM could probably be reduced further by ensuring that stools were not inoculated too heavily on CSM. Our findings confirm that charcoal is an effective substitute for blood in media for growing campylobacters, and that CSM is a highly effective blood-free selective medium for isolating C. jejuni and C. coli from stools.
机译：一种无血，基于木炭的选择性培养基（CSM），由哥伦比亚琼脂碱，活性炭（4 g /升），血红素（0.032 g /升），丙酮酸钠（0.1 g /升），头孢哌酮（32 mg）组成/升），万古霉素（20毫克/升）和环己酰亚胺（100毫克/升）支持空肠弯曲菌和大肠杆菌的生长，其菌落数与无抗生素马血琼脂的菌落数相同。将CSM与Skirrow培养基（SKM）进行比较，以从腹泻患者的粪便中回收空肠弯曲杆菌和大肠杆状杆菌，将其在43摄氏度的氧气压力降低的情况下孵育2天。在1,227份粪便中进行了2.9％的检测（两种培养基中29种，仅CSM中5种，SKM中一种）。每当回收空肠弯曲杆菌和大肠杆菌时，在29种CSM培养物中（85％）的生长都是纯的，而在11种SKM培养物中（37％）的生长是纯的。与426种SKM培养物（35％）相比，在704种CSM培养物中（57％）完全抑制了“污染”菌群。与SKM相比，CSM更有效地抑制了污染的假单胞菌，革兰氏阳性生物和酵母菌。两种培养基都未能抑制大约四分之一的样品中肠杆菌科的成员。对20种代表性肠杆菌科细菌的研究表明，对头孢哌酮的敏感性和CSM的生长显着取决于接种物的大小。头孢哌酮（32毫克/升）以5 X 10（2）和5 X 10（4）的菌体大小抑制了12株，但没有5 X 10（6）的菌体被抑制，这表明CSM上的污染物频率可能通过确保粪便未过度接种CSM来进一步减少粪便。我们的发现证实，木炭可以有效替代生长中弯曲杆菌的培养基中的血液，而CSM是从粪便中分离空肠弯曲杆菌和大肠杆菌的高效无血选择性培养基。

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