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首页> 外文期刊>Journal of Clinical Microbiology >Chromatofocusing in the purification of staphylococcal enterotoxin D.
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Chromatofocusing in the purification of staphylococcal enterotoxin D.

机译:色谱聚焦在葡萄球菌肠毒素D纯化中。

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A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.
机译:建立了用于金黄色葡萄球菌肠毒素D纯化的色谱聚焦程序。纯化包括通过用CG-50树脂分批吸附从金黄色葡萄球菌1151m的培养上清液中除去有毒蛋白质,在Polybuffer Exchanger 94上进行色谱聚焦,并在Sephacryl S-200上进行凝胶渗透色谱。获得的葡萄球菌肠毒素D的纯度约为98%。

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