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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of rotavirus immunoglobulin A coproconversion with other indices of rotavirus infection in a longitudinal study in childhood.
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Comparison of rotavirus immunoglobulin A coproconversion with other indices of rotavirus infection in a longitudinal study in childhood.

机译：轮状病毒免疫球蛋白A与儿童轮状病毒感染其他指标的共转化的比较在一项儿童期纵向研究中。

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In order to determine the sensitivity and reliability of antirotaviral fecal immunoglobulin A (IgA) as an indicator of rotavirus reinfection, the antibody responses to rotavirus of 44 infants with severe rotavirus gastroenteritis recruited on admission to a hospital were studied. Feces were collected daily during hospitalization and weekly thereafter, and sera were obtained every 4 months, for 6 to 32 months (median, 17 months). Antirotaviral IgG, IgA, and IgM were measured by enzyme immunoassay in all samples. Rotavirus antigen, rotavirus-neutralizing antibody, and total IgA were measured in feces. The results showed that use of an IgA index (ratio of specific IgA to total IgA) was unnecessary to identify copro-IgA conversion to rotavirus. The other markers of rotavirus infection tested showed a high level of predictive accuracy of coproconversion in rotavirus-neutralizing antibody. Copro-IgM, serum IgM, and virus in feces were insensitive measures of neutralizing antibody coproconversion. Seroconversion in IgG or IgA was detected in 46% of neutralizing coproconversions. The most sensitive marker, present in 92% of neutralizing coproconversions, was antirotaviral fecal IgA conversion. This correlation of fecal IgA with fecal neutralizing antibody suggests that coproconversions in IgA represent true elevations in antirotaviral IgA with neutralizing capacity. A coproconversion in IgA appears to indicate genuine rotavirus infection. Copro-IgA conversions in feces collected weekly are likely to be more sensitive markers of rotavirus reinfection than are seroconversion and virus detection combined in epidemiological studies of acute diarrhea in children and in rotavirus vaccine trials.
机译：为了确定抗轮状病毒粪便免疫球蛋白A（IgA）作为轮状病毒再感染指标的敏感性和可靠性，研究了入院时招募的44例患有严重轮状病毒胃肠炎的婴儿对轮状病毒的抗体反应。住院期间每天收集粪便，此后每周收集一次，每4个月收集一次血清，持续6至32个月（中位数为17个月）。通过酶免疫测定法对所有样品中的抗轮状病毒IgG，IgA和IgM进行​​了测定。在粪便中测量轮状病毒抗原，轮状病毒中和抗体和总IgA。结果表明，无需使用IgA指数（特定IgA与总IgA之比）来确定copro-IgA向轮状病毒的转化。测试的轮状病毒感染的其他标志物显示出在轮状病毒中和抗体中共促转化的预测准确性很高。粪便中的Copro-IgM，血清IgM和病毒是中和抗体coproconversion的不敏感措施。在46％的中和共原转化中检测到IgG或IgA的血清转化。在92％的中和共前转化中，最敏感的标志物是抗旋转病毒粪便IgA转化。粪便中IgA与粪便中和抗体的这种相关性表明，IgA中的共转化代表具有中和能力的抗旋转病毒IgA的真实升高。 IgA中的coproconversion似乎表明真正的轮状病毒感染。在儿童急性腹泻的流行病学研究和轮状病毒疫苗试验中，每周收集的粪便中Copro-IgA转化可能是轮状病毒再感染的更敏感标志，而不是血清转化和病毒检测。

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