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首页> 外文期刊>Journal of Clinical Microbiology >Immunodiffusion test for serodiagnosing subcutaneous zygomycosis.
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Immunodiffusion test for serodiagnosing subcutaneous zygomycosis.

机译：免疫扩散试验用于血清诊断皮下合子菌病。

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摘要

Culture filtrate antigens of Basidiobolus ranarum and Conidiobolus coronatus were analyzed by immunodiffusion (ID) with homologous rabbit antisera. B. ranarum and C. coronatus were each found to have five specific antigens. Results of tests with heterologous antisera indicated that all of the species shared at least one antigen. ID tests incorporating the specific precipitin bands as references were developed for detection of basidiobolomycosis and conidiobolomycosis. These tests were performed with sera from humans and horses with proven basidiobolomycosis and conidiobolomycosis as well as with control sera from humans and animals with and without heterologous mycotic and oomycotic infections. Only sera from cases of basidiobolomycosis and conidiobolomycosis produced lines of identity with the reference precipitates of B. ranarum and C. coronatus, respectively. The ID tests were found to be completely sensitive and specific for determining the etiology of zygomycosis caused by these two species. In addition they appeared useful for monitoring resolution of the infections.
机译：用同源兔抗血清通过免疫扩散法（ID）分析了Basidiobolus ranarum和Coroniobolus coronatus的培养滤液抗原。发现雷氏芽孢杆菌和冠状梭菌分别具有五个特异性抗原。异源抗血清的测试结果表明，所有物种均共享至少一种抗原。开发了结合特定沉淀蛋白条带作为参考的ID测试，用于检测巴斯德菌病和分生孢子菌病。这些测试是用人和马的血清和已证实的芥子菌病和分生孢子菌的血清，以及人和动物有或没有异源真菌和卵菌感染的对照血清进行的。仅来自根瘤菌病和分生孢子菌病病例的血清分别与雷氏芽孢杆菌和冠状隐孢子虫的参考沉淀物产生同一系。发现ID测试对于确定由这两个物种引起的合子菌病的病因学是完全敏感和特异性的。另外，它们似乎对于监测感染的消退很有用。

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