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首页> 外文期刊>Journal of Clinical Microbiology >Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains.
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Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains.

机译：种类，生物型和噬菌体类型测定与用于不动杆菌菌株分型的细胞包膜蛋白谱进行比较。

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摘要

Species, biotypes, and phage types were determined for 120 Acinetobacter strains from clinical or environmental sources or from culture collections. These characteristics were compared with cell envelope protein profiles obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in previous studies. A considerable heterogeneity of species and types was observed by use of the various methods, in particular among strains from different sources. Acinetobacter baumannii was the most commonly found species in isolates from clinical sources, followed by Acinetobacter species 3. Nine biotypes were observed among A. baumannii strains. Further differentiation within most species and biotypes was achieved by protein profile typing and, to some extent, phage typing. Of 120 strains, 49 (41%) were not typeable by phages. Consistent results for the various classification methods were obtained for strains from common sources. Biotyping seemed an appropriate method for the screening of strains, whereas protein profile and phage typing could serve as additional methods to establish the identity or nonidentity of strains. Results of this study suggest that the combination of the typing methods is useful in epidemiological studies.
机译：从临床或环境来源或从培养物收集物中确定了120种不动杆菌的种类，生物型和噬菌体类型。将这些特性与通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳获得的细胞包膜蛋白谱进行比较。通过使用各种方法，尤其是在不同来源的菌株中，观察到物种和类型的相当大的异质性。鲍曼不动杆菌是临床来源分离物中最常见的物种，其次是不动杆菌属3。在鲍曼不动杆菌菌株中观察到9种生物型。在大多数物种和生物型中，进一步的分化是通过蛋白质谱型分型和某种程度上的噬菌体型实现的。在120个菌株中，有49个（41％）无法通过噬菌体分型。对于来自共同来源的菌株，获得了各种分类方法的一致结果。生物分型似乎是筛选菌株的合适方法，而蛋白质谱和噬菌体分型可以作为确定菌株身份或非身份的其他方法。这项研究的结果表明，分型方法的组合在流行病学研究中很有用。

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