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首页> 外文期刊>Journal of Clinical Microbiology >Enzymatic RNA amplification of the enteroviruses.
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Enzymatic RNA amplification of the enteroviruses.

机译：肠病毒的酶促RNA扩增。

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Enteroviruses are among the most common causes of childhood infection. Current diagnostic techniques are often too slow and too insensitive to benefit the patient optimally. This report describes a modified polymerase chain reaction technique by which enteroviral RNA can be amplified, over a few hours, to a level detectable by agarose mini-gel electrophoresis or nucleic acid hybridization or both. Three oligomeric regions of great homology among the enteroviruses were identified and designated as a potential primer pair and probe. With this combination, all 11 of the enterovirus serotypes tested, representing the major subgroups of these pathogens, were successfully amplified and detected. The sensitivity and rapidity of this new assay speak to its potential clinical applicability in the diagnosis of enterovirus infections.
机译：肠病毒是儿童感染的最常见原因。当前的诊断技术通常太慢并且太不灵敏以至于不能使患者受益。该报告描述了一种改良的聚合酶链反应技术，通过该技术，可以在数小时内将肠病毒RNA扩增到琼脂糖微凝胶电泳或核酸杂交或两者均可检测到的水平。肠病毒中三个具有高度同源性的寡聚区被鉴定为潜在的引物对和探针。通过这种组合，成功地扩增和检测了代表这些病原体主要亚群的所有11种肠病毒血清型。这种新方法的灵敏性和快速性证明了其在肠病毒感染诊断中的潜在临床适用性。

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