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首页> 外文期刊>Journal of Clinical Microbiology >Analyses of the FlashTrack DNA probe and UTIscreen bioluminescence tests for bacteriuria.
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Analyses of the FlashTrack DNA probe and UTIscreen bioluminescence tests for bacteriuria.

机译：用于细菌尿的FlashTrack DNA探针分析和UTIscreen生物发光测试。

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Five hundred urine specimens were selected at random and screened for bacteriuria by a DNA probe method, FlashTrack (Gen-Probe, San Diego, Calif.), and an automated bioluminescence method, UTIscreen (Los Alamos Diagnostics, Los Alamos, N.M.), and the results were compared with those of the semiquantitative plate culture method. The performance of each test versus culturing was evaluated at colony counts of greater than or equal to 10(4), greater than or equal to 5 x 10(4), and greater than or equal to 10(5) CFU/ml. Since the interpretive breakpoint of each test was user selectable, the results were reported as receiver operator characteristic curves. Optimum interpretive breakpoints were determined for each test at each colony count by calculating a performance index that emphasized sensitivity over specificity in a 70:30 ratio. Although both tests had less-than-optimal sensitivities and specificities, the performance of FlashTrack was significantly better than that of UTIscreen at two of the three colony counts (10(4) and 10(5) CFU/ml); however, FlashTrack costs more and is a labor-intensive procedure. Neither method was evaluated for the detection of colony counts of less than 10(4) CFU/ml.
机译：随机选择500个尿液标本，并通过DNA探针法FlashTrack（Gen-Probe，San Diego，CA）和自动生物发光法UTIscreen（Los Alamos Diagnostics，Los Alamos，NM）筛选细菌尿。将结果与半定量平板培养法进行了比较。在集落数大于或等于10（4），大于或等于5 x 10（4）和大于或等于10（5）CFU / ml的情况下评估每个测试相对于培养的性能。由于每个测试的解释性断点是用户可选的，因此将结果报告为接收器操作员特征曲线。通过计算性能指标，以70:30的比例强调灵敏度高于特异性，确定了每个菌落计数下每个测试的最佳解释性断裂点。尽管这两个测试的灵敏度和特异性均未达到最佳，但在三个菌落计数中的两个（10（4）和10（5）CFU / ml）中，FlashTrack的性能明显优于UTIscreen。但是，FlashTrack成本更高，并且是一项劳动密集型过程。两种方法均未评估菌落计数低于10（4）CFU / ml的检测方法。

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