...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Development and evaluation of PCR assay for detection of low levels of Cowdria ruminantium infection in Amblyomma ticks not detected by DNA probe.
【24h】

Development and evaluation of PCR assay for detection of low levels of Cowdria ruminantium infection in Amblyomma ticks not detected by DNA probe.

机译：PCR检测试剂盒的开发和评估，用于检测DNA探针未检测到的羊膜滴虫中低水平的反刍动物反刍动物感染。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The sensitivities of a PCR assay and a DNA probe assay were compared for the detection of Cowdria ruminantium in Amblyomma ticks that were fed on C. ruminantium-infected, clinically reacting, and recovered carrier animals. The PCR assay and DNA probe detected infection in 86.0 and 37.0%, respectively, of 100 ticks fed on a febrile animal. In 75 ticks fed on carrier animals, PCR and the DNA probe detected infection in 28.0 and 1.33% of ticks, respectively. This demonstrates that the DNA probe has poor sensitivity for the detection of low levels of infection in ticks and that PCR is necessary for this purpose. The PCR assay had a detection limit of between 1 and 10 C. ruminantium organisms and did not amplify DNA from Ehrlichia canis, which is phylogenetically closely related to C. ruminantium, Theileria parva, or uninfected Amblyomma hebraeum or A. variegatum. PCR detected infection in A. hebraeum and A. variegatum adult ticks infected with one of six geographically different C. ruminantium strains. Amplification was also possible from desiccated ticks and ticks fixed in 70% ethanol, 10% buffered formalin, or 2% glutaraldehyde. The PCR assay supersedes the DNA probe and older detection methods for the detection of C. ruminantium in ticks, particularly those fed on carrier animals, and is suitable for both prospective and retrospective studies which require accurate detection of C. ruminantium in individual ticks. Application of the PCR assay should significantly improve the understanding of heartwater epidemiology, particularly through the determination of field tick infection rates.
机译：比较了PCR测定法和DNA探针测定法的敏感性，以检测饲喂反刍动物感染，临床反应并已恢复的带菌动物的A中的反刍动物Cow。 PCR检测和DNA探针分别在饲养高热动物的100只s中检测到感染的86.0％和37.0％。在以食肉动物为食的75个tick中，PCR和DNA探针分别在28.0％和1.33％的s中检测到感染。这表明DNA探针对tick中低水平感染的检测灵敏度较差，因此PCR是必需的。 PCR检测的检出限为1到10个反刍动物微生物，并且未扩增犬埃里希氏体的DNA，而Ehrlichia canis在系统发育上与反刍动物克鲁维氏菌，小花泰勒虫或未感染的盲mb或杂色曲霉密切相关。 PCR检测到感染了六个地理上不同的反刍类反刍动物菌株之一的希伯来曲霉和杂色曲霉成年tick。干燥的s和固定在70％乙醇，10％缓冲福尔马林或2％戊二醛中的tick也可以扩增。 PCR测定法取代了DNA探针和较旧的检测方法，用于检测tick中的反刍动物，特别是饲喂携带动物的反刍动物，适用于前瞻性研究和回顾性研究，这些研究需要准确检测单个tick中的反刍动物。 PCR检测的应用应显着改善对心脏水流行病学的了解，特别是通过确定田tick感染率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号