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首页> 外文期刊>Journal of Clinical Microbiology >Detection and characterization of antigens in urine of patients with acute, congenital, and chronic Chagas' disease.
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Detection and characterization of antigens in urine of patients with acute, congenital, and chronic Chagas' disease.

机译：患有急性，先天性和慢性恰加斯病的患者尿液中抗原的检测和表征。

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Monoclonal antibodies raised against purified Trypanosoma cruzi urinary antigens were used in an enzyme-linked immunosorbent assay (ELISA) capture test for parasite antigens present in urine specimens of Argentinean and Brazilian patients with Chagas' disease. At diagnosis, antigenuria was demonstrated by ELISA in all acutely and congenitally infected infants studied. Moreover, T. cruzi urinary antigens were detected in samples from three of five patients with acute infections and four of five patients with congenital infections following chemotherapy. At least one ELISA-positive urine specimen from each individual was recorded in a longitudinal survey of 12 chronic chagasic patients. The same parasitic antigens (90 to 80 kDa, pI 5.7 to 6.0; 70 to 65 kDa, pI 4.9 to 4.5; 50 to 45 kDa, pI 5.3 to 5.1; and 40 to 35 kDa, pI 4.8 to 4.5) were identified by immunoprecipitation and two-dimensional polyacrylamide gel electrophoresis analysis of urine samples from patients with different forms of chagasic infection. The 90- to 80-kDa urinary protein resembles a trypomastigote-shed antigen. Determination of antigenuria proved valuable for early diagnosis of Chagas' disease and also for diagnosis of chronic cases with conflicting serology.
机译：针对纯化的克鲁斯锥虫泌尿抗原产生的单克隆抗体被用于酶联免疫吸附测定（ELISA）捕获测试中，用于检测阿根廷和巴西恰加斯病患者尿液样本中存在的寄生虫抗原。诊断时，所有研究的急性和先天感染婴儿均通过ELISA证实了尿蛋白尿。此外，在化疗后，从五分之三的急性感染患者和五分之四的先天性感染患者的样品中检出了克鲁氏锥虫尿抗原。在对12位慢性Chachaic患者的纵向调查中，记录了每个个体的至少一个ELISA阳性尿液样本。通过免疫沉淀鉴定出相同的寄生抗原（90至80 kDa，pI 5.7至6.0; 70至65 kDa，pI 4.9至4.5; 50至45 kDa，pI 5.3至5.1;以及40至35 kDa，pI 4.8至4.5）。和二维聚丙烯酰胺凝胶电泳分析不同形式的chagasic感染患者的尿液样品。 90-80kDa的尿蛋白类似于胰杆后鞭毛脱落的抗原。抗原尿的测定被证明对查加斯病的早期诊断以及血清学冲突的慢性病例的诊断很有价值。

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