Intravitam Diagnosis of Human Rabies by PCR Using Saliva and Cerebrospinal Fluid

P. Crepin; L. Audry; Y. Rotivel; A. Gacoin; C. Caroff; H. Bourhy

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Intravitam Diagnosis of Human Rabies by PCR Using Saliva and Cerebrospinal Fluid

机译：唾液和脑脊液PCR检测人类狂犬病的体内诊断

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An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.

机译：优化的逆转录（RT）-PCR方案，用于从狂犬病病毒基因组RNA的体内检测，使用从28名怀疑患有狂犬病的患者中获得的临床样本进行测试，其中9名通过验尸证实患有狂犬病。使用唾液的RT-PCR结合对皮肤活检样品进行的免疫荧光测定可以检测9名患者的狂犬病。

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《Journal of Clinical Microbiology》 |1998年第4期|共5页
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P. Crepin; L. Audry; Y. Rotivel; A. Gacoin; C. Caroff; H. Bourhy;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Real-time PCR analysis of dog cerebrospinal fluid and saliva samples for ante-mortem diagnosis of rabies. [J] . Saengseesom W, Mitmoonpitak C, Kasempimolporn S, The Southeast Asian journal of tropical medicine and public health . 2007,第1期

机译：实时PCR分析狗脑脊液和唾液样本，以进行狂犬病事前诊断。
2. Sensitivity comparison of nested RT-PCR and TaqMan real time PCR for intravitam diagnosis of rabies in animals from urine samples [J] . M. Dandale, C. K. Singh, V. Ramneek, Veterinary World . 2013,第4期

机译：巢式RT-PCR和TaqMan实时PCR在尿液中动物体内狂犬病的体内诊断的敏感性比较
3. Utility of rabies neutralizing antibody detection in cerebrospinal fluid and serum for ante-mortem diagnosis of human rabies [J] . Tina Damodar, Reeta S. Mani, P. V. Prathyusha PLOS Neglected Tropical Diseases . 2019,第1期

机译：脑脊液和血清中狂犬病中和抗体检测在人类狂犬病事前诊断中的实用性
4. Low-dose intrathecal fluorescein for diagnosis of cerebrospinal fluid rhinorrhea using the scanning fiber endoscope in the human nasal cavities [C] . Vivian W. Hou, Calvin G. Davis, Greg E. Davis, SPIE Conference on Optical Diagnostics and Sensing . 2016

机译：低剂量鞘内荧光荧光素用于使用扫描纤维内窥镜在人鼻腔中诊断脑脊液鼻窦
5. Microbial forensics and the use of Rt-PCR and NASBA for human saliva evidence analysis [D] . Counsil, Tyler Isaac 2011

机译：微生物取证以及Rt-PCR和NASBA在人类唾液证据分析中的应用
6. Note: Intravitam Diagnosis of Human Rabies by PCR Using Saliva and Cerebrospinal Fluid [O] . P. Crepin, L. Audry, Y. Rotivel, 1998

机译：注意：使用唾液和脑脊液通过PCR对人的狂犬病进行体内诊断
7. Sensitivity comparison of nested RT-PCR and TaqMan real time PCR for intravitam diagnosis of rabies in animals from urine samples [O] . N. K. Sood, D. Deka, K. Bansal, 2013

机译：巢式RT-pCR和Taqman实时pCR的灵敏度比较，用于尿样动物狂犬病的玻璃体内诊断

Intravitam Diagnosis of Human Rabies by PCR Using Saliva and Cerebrospinal Fluid

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