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首页> 外文期刊>Journal of Clinical Microbiology >Effects of Decontamination Methods and Culture Conditions on Viability of Mycobacterium ulcerans in the BACTEC System
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Effects of Decontamination Methods and Culture Conditions on Viability of Mycobacterium ulcerans in the BACTEC System

机译：净化方法和培养条件对BACTEC系统中溃疡分枝杆菌活力的影响

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We used the BACTEC system to evaluate the effects of several decontamination methods and the addition of antibiotics on the viability of Mycobacterium ulcerans. The effects of polyoxyethylene stearate or egg yolk as supplements were also evaluated to determine their impact on the growth of M. ulcerans. Strains of different geographic origins were subjected to Petroff, reversed Petroff, oxalic acid, and mild HCl treatments. After treatment, the viability of each strain was assessed in the BACTEC system. All of the decontamination methods tested adversely affected bacterial viability. Treatment with mild HCl gave the best results, allowing better growth rates with some strains and causing a delay in growth with others, depending on the geographic origin of the strain. A mixture of polymyxin B, amphotericin B, nalidixic acid, trimethoprim, and azlocillin did not significantly inhibit growth. Supplementing BACTEC medium with egg yolk markedly improved the recovery of M. ulcerans following the use of each of the decontamination methods. Our findings demonstrate a detrimental impact on the viability of M. ulcerans by all of the decontamination methods currently in common use. This explains, at least in part, the difficulty often experienced in cultivating this organism from clinical specimens. Egg yolk should be added to enhance the rate of successful primary cultivation of M. ulcerans in the BACTEC system.
机译：我们使用BACTEC系统评估了几种去污方法和添加抗生素对溃疡分枝杆菌（emcobacterium ulcerans）生存力的影响。还评估了聚氧乙烯硬脂酸酯或蛋黄作为补充剂的作用，以确定它们对 M生长的影响。溃疡。对不同地理起源的菌株进行了彼得罗夫，反向彼得罗夫，草酸和温和的HCl处理。处理后，在BACTEC系统中评估每种菌株的生存力。测试的所有去污方法均对细菌生存能力产生不利影响。根据菌株的地理来源，用温和的HCl处理可获得最佳效果，某些菌株的生长速度更快，而另一些菌株的生长延迟。多粘菌素B，两性霉素B，萘啶酸，甲氧苄啶和阿洛西林的混合物没有明显抑制生长。用蛋黄补充BACTEC培养基可显着提高 M的回收率。每种去污方法的使用会导致溃疡。我们的发现表明对 M的生存力有不利影响。溃疡通过目前所有常用的去污方法进行。这至少部分地解释了从临床标本中培养这种生物体经常遇到的困难。应该添加蛋黄以提高 M的成功初次培养率。 BACTEC系统中的溃疡。

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