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首页> 外文期刊>Journal of Clinical Microbiology >Microaerophilic Conditions Promote Growth ofMycobacterium genavense
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Microaerophilic Conditions Promote Growth ofMycobacterium genavense

机译：微需氧条件促进genavenbacterium genavense的生长。

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Our studies show that microaerophilic conditions promote the growth of Mycobacterium genavense in semisolid medium. The growth of M. genavense at 2.5 or 5% oxygen was superior to that obtained at 21% oxygen in BACTEC primary cultures (Middlebrook 7H12, pH 6.0, without additives). By using nondecontaminated specimens, it was possible to detect growth with very small inocula (25 bacilli/ml) of 12 different M. genavense strains (from nude mice) within 6 weeks of incubation under low oxygen tension; conversely, with 21% oxygen, no growth of 8 of 12 (66.7%) M. genavense strains was detected (growth index, <10). The same beneficial effect of 2.5 or 5% oxygen was observed in primary cultures of a decontaminated clinical specimen. Low oxygen tension (2.5 or 5%) is recommended for the primary isolation ofM. genavense. Microaerophilic cultivation of other atypical mycobacteria, especially slow-growing (e.g., Mycobacterium avium) and difficult-to-grow (e.g., Mycobacterium ulcerans) species, is discussed.
机译：我们的研究表明，微需氧条件可促进半固体培养基中的Genem分支杆菌的生长。 M的增长。在2.5％或5％氧气条件下的Genavense 优于在BACTEC原始培养物中（Middlebrook 7H12，pH 6.0，不含添加剂）的情况。通过使用未消毒的标本，可以使用12种不同的M的极小接种量（25细菌/毫升）检测生长。在低氧压力下孵育6周内的genavense菌株（来自裸鼠）;相反，在含21％氧气的情况下，没有12个中的8个（66.7％）M的生长。检测到genavense 菌株（生长指数，<10）。在去污的临床标本的原代培养物中观察到了2.5或5％氧气的相同有益效果。建议将低氧压（2.5或5％）用于 M的初步隔离。 genavense 。讨论了其他非典型分枝杆菌的微需氧培养，尤其是缓慢生长的（例如分枝杆菌）和难以生长的（例如分枝杆菌）物种。

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