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首页> 外文期刊>Journal of Clinical Microbiology >Visualization of Hydatid Elements: Comparison of Several Techniques
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Visualization of Hydatid Elements: Comparison of Several Techniques

机译：Hy虫元素的可视化：几种技术的比较

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Some techniques available at our laboratory were tested for their ability to aid in the morphological diagnosis of hydatid elements (Echinococcus granulosus [“Taenia echinococcus”]) isolated from cysts in humans and sheep. Unstained, methanol-fixed hooklets were fluorescent, most starkly so under violet light (excitation filter wavelength, 405 nm; long-pass filter wavelength, 495 nm). Auramine-rhodamine and Gram procedures failed to stain hooklets. Ziehl-Neelsen stain yielded indifferent results when organisms were viewed under transmitted light but resulted in a surprisingly intense red fluorescence when organisms were viewed under green light (excitation, 546 nm; long pass, 590 nm). Wheatley trichrome stain gave better and more uniform results than fuchsin. Ryan trichrome blue stain was the best under transmitted light; hooklets stained uniformly and intensely and were easily distinguishable from the background. Very satisfactory results were also obtained with a much simpler procedure (modified Baxby technique: no fixation, steaming hot 1% safranin for 2 min, and malachite green for 30 s). Therefore, Ryan and modified Baxby stains are recommended for the examination of E. granulosus under transmitted light. For fluorescence microscopy, Ziehl-Neelsen stain under green excitation light, or violet light with no staining, is also very useful. Epifluorescence microscopy is especially convenient for examining samples concentrated by filtration, as it renders the filter pores inconspicuous.
机译：对我们实验室中可用的一些技术进行了测试，这些技术有助于从人和女性的囊肿中分离出的包虫元素（ Echinococcus granulosus [“ Taenia echinococcus ”]）进行形态学诊断羊。未染色的，甲醇固定的钩状荧光素，发荧光，在紫光（激发滤光片波长为405 nm;长通滤光片波长为495 nm）下最为明显。 Auramine-rhodamine和Gram程序无法染色钩状小钩。当在透射光下观察生物时，Ziehl-Neelsen染色产生的结果不显着，但在绿光下（激发，546 nm;长通，590 nm）观察，则产生令人惊讶的强烈红色荧光。惠特利三色染色剂比品红具有更好，更均匀的效果。莱恩三色蓝染色在透射光下效果最好;小钩被均匀且强烈地染色，很容易与背景区分开。用更简单的方法（改进的Baxby技术：不固定，热1％番红花蒸2分钟和孔雀石绿蒸30秒）也获得了非常令人满意的结果。因此，建议将Ryan和改良的Baxby染色剂用于 E的检查。透射光下的颗粒。对于荧光显微镜，在绿色激发光或没有染色的紫光下的Ziehl-Neelsen染色也是非常有用的。落射荧光显微镜特别方便检查通过过滤浓缩的样品，因为它使过滤器的孔不明显。

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