...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Nasopharyngeal Carriage of Penicillin-ResistantStreptococcus pneumoniae among Children with Acute Respiratory Tract Infections in Thailand: a Molecular Epidemiological Survey
【24h】

Nasopharyngeal Carriage of Penicillin-ResistantStreptococcus pneumoniae among Children with Acute Respiratory Tract Infections in Thailand: a Molecular Epidemiological Survey

机译：在泰国患有急性呼吸道感染的儿童中耐青霉素肺炎链球菌的鼻咽支架：分子流行病学调查

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The prevalence of penicillin-resistant Streptococcus pneumoniae in Thailand has dramatically increased over the last decade. During a national survey, which was conducted from 1992 to 1994, 37.2% of the pneumococci isolated from the nasopharynges of children with acute respiratory tract infections were penicillin resistant (MIC, ≥0.1 μg/ml). In order to investigate the prevalence and clonal relatedness of nasopharyngeal carriage of penicillin-resistant S. pneumoniae in Thailand, a molecular epidemiological survey was undertaken. To this end, 53 penicillin-resistant pneumococcal isolates from children who suffered from acute respiratory tract infections and who originated from five distinct regions of the country were characterized in detail. DNA fingerprint analysis demonstrated 13 clusters, i.e., genotypes shared by two or more strains, and 14 unique genotypes. The cluster size varied from 2 (nine clusters) to 11 strains (one cluster). Six of the 13 restriction fragment end labeling clusters consisted of two or more distinct serotypes, indicating frequent horizontal transfer of capsular genes. Geographical distribution of the genotypes among the five regions of Thailand demonstrated that only four genetic clusters were restricted to single areas of the country, whereas the other nine clusters represented isolates collected in two or more districts. These observations demonstrate that the majority of the genetic clusters are spread throughout the country. The most predominant genetic cluster, representing 21% of the isolates, was identical to the Spanish pandemic clone 23F. In addition, the second largest cluster matched the Spanish-French international clone 9V. These data indicate that the genetic clones 23F and 9V, which are widely spread throughout the world, are the most predominant multidrug-resistant pneumococcal clones in Thailand. Therefore, we conclude that these pandemic clones are primarily responsible for the increase in the prevalence of pneumococcal penicillin resistance in Thailand.
机译：在过去十年中，泰国对青霉素耐药的肺炎链球菌的患病率急剧上升。在1992年至1994年进行的一项全国调查中，从患有急性呼吸道感染的儿童鼻咽中分离出的肺炎球菌中有37.2％对青霉素耐药（MIC，≥0.1μg/ ml）。为了调查耐青霉素 S的鼻咽运输的流行和克隆相关性。在泰国对肺炎进行了分子流行病学调查。为此，对来自急性呼吸道感染并来自该国五个不同地区的儿童的53株抗青霉素肺炎球菌进行了详细鉴定。 DNA指纹分析显示了13个簇，即两个或多个菌株共有的基因型，以及14个独特的基因型。簇的大小从2个（九个簇）到11个菌株（一个簇）不等。 13个限制性片段末端标记簇中的6个由两种或更多种不同的血清型组成，表明荚膜基因频繁水平转移。该基因型在泰国五个地区之间的地理分布表明，只有四个基因簇仅限于该国的单个地区，而其他九个簇代表了在两个或多个地区收集的分离株。这些观察表明，大多数遗传簇分布在全国各地。最主要的遗传簇，占分离株的21％，与西班牙的大流行克隆23F相同。此外，第二大集群与西班牙-法国国际克隆9V相匹配。这些数据表明，在世界范围内广泛分布的基因克隆23F和9V是泰国最主要的耐多药肺炎球菌克隆。因此，我们得出结论，这些大流行性克隆主要是导致泰国肺炎球菌青霉素耐药性增加的原因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号