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首页> 外文期刊>Journal of Clinical Microbiology >Immunoglobulin-specific radioimmunoprecipitation assays for quantitation of nasal secretory antibodies to hemagglutinin of type A influenza viruses.
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Immunoglobulin-specific radioimmunoprecipitation assays for quantitation of nasal secretory antibodies to hemagglutinin of type A influenza viruses.

机译：免疫球蛋白特异性放射免疫沉淀测定法，用于定量检测A型流感病毒血凝素的鼻分泌抗体。

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摘要

Radioimmunoprecipitation (RIP) assays were developed to selectively quantitate class-specific antibodies to purified hemagglutinins (HA) of type A influenza virus in nasal secretions. Rabbit anti-human secretory piece of immunoglobulin A (IgA) and rabbit anti-human IgG were used as second antibodies. A third antibody, goat anti-rabbit IgG, was incorporated into the system to separate immune complexes formed between iodinated HA, nasal wash test specimen, and second antibody. The utilization of this reagent avoided the need for large quantities of IgA and IgG antibody-negative carrier secretions. Nasal was specimens obtained from 14 adults immunized with an inactivated type A influenza virus vaccine were evaluated by RIP and viral neutralization assays. Significant homologous postvaccination secretory IgA and IgG antibody levels were demonstrable in 13 (93%) of individuals by RIP, whereas only 5 (36%) exhibited rises by viral neutralization tests. Moreover, the geometric mean IgA and IgG antibody levels were at least 20- and 37-fold greater than the neutralizing antibody titer. The pattern of heterologous immunoglobulin-specific antibody responses tended to be similar to those observed with the homologous HA subunit.
机译：开发了放射免疫沉淀（RIP）检测方法，以选择性地定量针对鼻分泌物中A型流感病毒的纯化血凝素（HA）的类特异性抗体。兔抗人免疫球蛋白A（IgA）分泌片段和兔抗人IgG用作第二抗体。将第三种抗体山羊抗兔IgG掺入系统中，以分离碘化HA，洗鼻液和第二抗体之间形成的免疫复合物。该试剂的使用避免了对大量IgA和IgG抗体阴性载体分泌的需求。通过RIP和病毒中和分析评估从14例用灭活的A型流感病毒疫苗免疫的成年人中获得的鼻标本。通过RIP可以在13（93％）个人中显示出显着同源的疫苗接种后IgA和IgG抗体水平，而通过病毒中和测试仅显示5（36％）升高。此外，几何平均IgA和IgG抗体水平比中和抗体滴度至少高20倍和37倍。异源免疫球蛋白特异性抗体应答的模式倾向于与同源HA亚基观察到的模式相似。

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