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首页> 外文期刊>Journal of Clinical Microbiology >New, simple medium for selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces.
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New, simple medium for selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces.

机译：从人粪便中选择性回收肺炎克雷伯菌和产氧克雷伯菌的新型简单培养基。

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A culture medium was developed which selectively favored the growth of Klebsiella pneumoniae and Klebsiella oxytoca in Escherichia coli-rich fecal cultures, without the use of antibiotics. The discriminative capacity of this medium was based on the presence of only two carbon sources, citrate and inositol, which can be utilized by nearly all K. pneumoniae and K. oxytoca strains but not by E. coli. The medium consisted of Simmons citrate agar (SCA) with 1% inositol (SCAI). Klebsiella strains from fecal samples subcultured on SCAI grew unhampered as yellow, dome-shaped, often mucoid colonies, whereas E. coli appeared as tiny, watery colonies. Apart from some Enterobacter strains, no other types of bacteria were found to mimic the typical appearance of klebsiellae. Recovery experiments from stool samples revealed a limiting ratio of Klebsiella to E. coli of 1:10(6) or more when samples were plated on SCAI versus ratios of 1:10(2) to 1:10(3) on blood agar or Macconkey agar. Compared with an existing Klebsiella culture method, the combination of SCA and MacConkey-inositol-carbenicillin (MIC) agar, Klebsiella yields with SCAI were not lower than those with the combination of MIC and SCA. Furthermore, the efficiency of the SCAI method was twice that of the latter combination. The SCAI plate could be a valuable tool in studies on the epidemiology of K. pneumoniae and K. oxytoca, for example in nosocomial infections, especially those concerning immunocompromised patients.
机译：在不使用抗生素的情况下，开发了一种在富含大肠杆菌的粪便培养物中选择性促进肺炎克雷伯菌和产氧克雷伯菌生长的培养基。该培养基的判别能力是基于仅柠檬酸和肌醇这两种碳源的存在，几乎所有肺炎克雷伯菌和产氧克雷伯菌都可以利用，但大肠杆菌不能利用。培养基由含1％肌醇（SCAI）的柠檬酸西蒙斯琼脂（SCA）组成。在SCAI上继代培养的粪便样本中的克雷伯菌菌株无阻碍地生长为黄色，圆顶形，通常为粘液样菌落，而大肠杆菌则显示为微小的水样菌落。除某些肠杆菌菌株外，未发现其他类型的细菌可模仿克雷伯氏菌的典型外观。从粪便样本中回收的实验表明，将样本涂在SCAI上时，克雷伯菌与大肠杆菌的极限比为1:10（6）或更高;而在血琼脂或大肠杆菌中，比例为1:10（2）至1:10（3）。麦康凯琼脂。与现有的克雷伯菌培养方法相比，SCA和MacConkey-肌醇-卡培尼西林（MIC）琼脂的组合，采用SCAI的克雷伯菌的产量均不低于MIC和SCA组合的克雷伯菌。此外，SCAI方法的效率是后者组合的两倍。 SCAI板可能是肺炎克雷伯菌和产氧克雷伯菌流行病学研究中的宝贵工具，例如在医院感染中，尤其是在免疫功能低下的患者中。

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