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首页> 外文期刊>Journal of Clinical Microbiology >HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand.
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HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand.

机译：从泰国腹泻婴儿中分离出的肠致病性大肠杆菌引起的HeLa细胞粘附和细胞毒素产生。

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Enteropathogenic Escherichia coli (EPEC) strains isolated from hospitalized infants with diarrhea in Thailand were examined for HeLa cell adherence and cytotoxin production. Of 101 strains examined, 56 adhered to HeLa cells in a localized pattern (LA), 27 adhered in a diffuse pattern (DA), and 18 did not adhere. All 56 LA EPEC strains were O:K serotype O119:K69. A total of 20 (83%) of 24 EPEC O86:K61 strains and 7 (38%) of 19 EPEC strains belonging to six other O:K serotypes exhibited DA. All LA EPEC strains hybridized with a DNA probe for genes encoding EPEC adherence factor, whereas none of the 27 DA or 18 nonadherent EPEC strains hybridized with EPEC adherence factor probe. Sonic extracts of 57 (58%) of 98 EPEC strains tested at a dilution of 1:100 caused greater than 25% mortality of HeLa cell monolayers. A total of 50 (88%) of 57 cytotoxic sonic extracts were inhibited to various degrees by a 1:500 dilution of polyclonal rabbit antisera to purified Shiga toxin. The mean percent inhibition of cytotoxic sonic extracts by anti-Shiga toxin was 67% (range, 29 to 89%). Fifty percent (38 of 56) of LA EPEC strains, fifty-two percent (14 of 27) of DA EPEC strains, and fifty-three percent (8 of 15) of nonadherent EPEC strains produced Shiga-like toxins. Both adherence and low levels of cell-associated cytotoxins were identified in EPEC strains from Thailand, but there did not appear to be an association between these two factors.
机译：从泰国住院的腹泻婴儿分离的肠致病性大肠杆菌（EPEC）菌株进行了HeLa细胞粘附和细胞毒素产生的检查。在检查的101个菌株中，有56个以局部模式（LA）粘附于HeLa细胞，27个以扩散模式（DA）粘附，而18个未粘附。所有56个LA EPEC菌株均为O：K血清型O119：K69。属于其他六种O：K血清型的24株EPEC O86：K61菌株中共有20株（83％）和19株EPEC菌株中的7株（38％）表现出DA。所有LA EPEC菌株均与DNA探针杂交以编码EPEC粘附因子基因，而27个DA或18个非粘附性EPEC菌株均未与EPEC粘附因子探针杂交。以1：100的稀释度测试的98种EPEC菌株中有57种（58％）的声波提取物导致HeLa细胞单层的死亡率超过25％。 1：500稀释的多克隆兔抗血清对纯净的志贺毒素的稀释，总共抑制了57种细胞毒性声音提取物中的50种（88％）。抗志贺毒素对细胞毒性声音提取物的平均抑制百分比为67％（范围为29％至89％）。 LA EPEC菌株中有百分之五十（56个中的38个），DA EPEC菌株中有52％（27个中的14个）和非粘附性EPEC菌株中有百分之五十三（15个中的8个）产生了志贺样毒素。在泰国的EPEC菌株中发现粘附和低水平的细胞相关细胞毒素，但是这两个因素之间似乎没有关联。

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