One hundred random urine specimens which were submitted for culture were planted using a calibrated loop within 2 h of collection and 2 and 4 h later after standing at room temperature. Colonies were counted after an 18- to 24-h incubation at 37 degrees C. Fifteen of the specimens demonstrated increases in counts exceeding 1 X log10; four increased from less than 10(5)/ml to greater than 10(5)/ml (three by the 4-h culture and a fourth by the 6-h culture), supporting the concept that delays of greater than 2 h in inoculating cultures may produce results which could cause errors in diagnosis.
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机译：在收集后的2小时内，室温下放置2和4小时后，使用校准环种植100个随机培养的尿液标本。在37摄氏度下孵育18至24小时后，对菌落进行了计数。其中有15个样本的计数超过1 X log10增加。四个从小于10（5）/ ml增加到大于10（5）/ ml（4小时培养3个，6小时培养4个），支持了延迟大于2 h的概念。接种培养物可能会产生可能导致诊断错误的结果。
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