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首页> 外文期刊>Journal of Clinical Microbiology >Diagnosis of acute toxoplasmosis by an enzyme immunoassay for specific immunoglobulin m antibodies.
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Diagnosis of acute toxoplasmosis by an enzyme immunoassay for specific immunoglobulin m antibodies.

机译：通过针对特定免疫球蛋白m抗体的酶免疫测定诊断急性弓形虫病。

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摘要

A recently developed enzyme-linked immunosorbent assay for detection of immunoglobulin M (IgM) class antibodies to Toxoplasma gondii was evaluated with respect to specificity and sensitivity. By using an antibody capture principle and F(ab')2 conjugates, interference of rheumatoid factors was absent. No cross-reactions with anti-toxoplasma IgG occurred, and no interference with antinuclear antibodies was found. A large-scale study with about 1,500 clinical specimens revealed a 100% specificity. By testing 79 sera from patients with acute-phase acquired toxoplasmosis, sensitivity was found to be 97%. In routine clinical practice, the IgM-enzyme-linked immunosorbent assay proved to be a more sensitive tool for diagnosis than the immunofluorescent-antibody test. The course of IgM-enzyme-linked immunosorbent assay antibodies in acute patients was studied; IgM reached peak levels within 1 month after onset of illness, and could be demonstrated up to an average of 8 months after onset.
机译：评估了针对弓形虫的免疫球蛋白M（IgM）类抗体的最新开发的酶联免疫吸附测定法的特异性和敏感性。通过使用抗体捕获原理和F（ab'）2共轭物，类风湿因子的干扰是不存在的。没有与抗弓形虫IgG发生交叉反应，也没有发现抗核抗体的干扰。一项包含约1,500个临床标本的大规模研究显示出100％的特异性。通过检测急性期获得性弓形虫病患者的79份血清，发现敏感性为97％。在常规临床实践中，与免疫荧光抗体测试相比，IgM酶联免疫吸附测定被证明是更敏感的诊断工具。研究了急性患者中IgM酶联免疫吸附测定抗体的过程; IgM在发病后1个月内达到峰值，并且可能在发病后平均8个月内得到证实。

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