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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of two transport systems for recovery of aerobic and anaerobic bacteria from abscesses.
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Comparison of two transport systems for recovery of aerobic and anaerobic bacteria from abscesses.

机译：比较两种从脓肿中回收需氧和厌氧细菌的运输系统。

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An evaluation of two commercially available swab transport systems, Port-A-Cul (PAC; BBL Microbiology Systems, Cockeysville, Md.) and Anaerobic Specimen Collector (ASC; Becton Dickinson Vacutainer Systems, Rutherford, N.J.), in the recovery of organisms from clinical specimens was done. Fifteen abscesses were drained, and swabs of their contents were placed in the transport systems until they were inoculated for detection of aerobic and anaerobic bacteria. The swabs were plated immediately after collection and after delays of 4, 24, 48, and 72 h. A total of 70 bacterial isolates, 47 anaerobes and 23 aerobes, were recovered from specimens inoculated immediately after collection. The survival of anaerobic bacteria was better in the PAC system than in the ASC system. This was evident as the length of delay in cultivation was extended. At 4 h, 46 anaerobic isolates were recovered in the PAC system, compared with 39 in the ASC system (P less than 0.1). At 24 h, 45 isolates were recovered in the PAC system and 26 isolates were recovered in ASC (P less than 0.001); at 48 h, 40 were recovered in PAC and 15 were recovered in ASC; and at 72 h, 32 were recovered in PAC and 6 were recovered in ASC. There were no differences between the systems in the recovery of aerobic bacteria. These data demonstrate the usefulness of the PAC system in the recovery of anaerobes and the need for quality control of all transport systems for anaerobic bacteria.
机译：对两种商业上可买到的拭子运输系统，Port-A-Cul（PAC; BBL Microbiology Systems，马里兰州科基斯维尔）和厌氧标本收集器（ASC; Becton Dickinson Vacutainer系统，NJ Rutherford，新泽西州）进行微生物回收的评估完成临床标本。排空十五个脓肿，并将其拭子放入运输系统中，直到接种好以检测需氧和厌氧细菌。拭子在收集后和延迟4、24、48和72小时后立即铺板。收集后立即接种的标本共回收了70种细菌分离株，47种厌氧菌和23种需氧菌。 PAC系统中厌氧细菌的存活率优于ASC系统。随着耕作延迟时间的延长，这一点显而易见。在4 h时，在PAC系统中回收了46种厌氧菌，而在ASC系统中则回收了39种（P小于0.1）。在24 h时，在PAC系统中回收了45个分离株，在ASC中回收了26个分离株（P小于0.001）。在48小时时，在PAC中回收了40个，在ASC中回收了15个;在第72小时，在PAC中回收了32个，在ASC中回收了6个。有氧细菌的回收率在系统之间没有差异。这些数据证明了PAC系统在厌氧菌回收中的有用性，以及对所有厌氧菌运输系统进行质量控制的需要。

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