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首页> 外文期刊>Journal of Clinical Microbiology >Restriction endonuclease analysis of nosocomial isolates of Clostridium difficile.
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Restriction endonuclease analysis of nosocomial isolates of Clostridium difficile.

机译：艰难梭菌医院分离株的限制性核酸内切酶分析。

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A total of 110 clinical isolates of Clostridium difficile were analyzed by agarose gel electrophoresis by using both bacterial restriction endonuclease analysis (REA) and plasmid profiles. A total of 72 isolates were divided into 12 groups according to their REA patterns. Some 38 isolates exhibited unique patterns. Pattern A occurred in 20% of isolates. Isolates with patterns B, E, and G were cytotoxin negative. The remaining groups were cytotoxin positive. Multiple isolates obtained from two stool specimens were studied to examine the variation in REA profiles found in single specimens. In these specimens no variation in REA profiles was found. The stability of C. difficile was studied by examining sequential in vitro subcultures of a single isolate and strains isolated over a 4-month period from two long-term carriers. REA patterns were stable over time, both in vitro and in vivo. Because plasmid DNA was observed in 53% of isolates, plasmid profiles alone could not be used to study the spread of C. difficile; however, they were necessary for the interpretation of REA patterns in some instances.
机译：使用细菌限制性核酸内切酶分析（REA）和质粒图谱，通过琼脂糖凝胶电泳对总共110株艰难梭菌临床分离株进行了分析。根据其REA模式，总共将72个分离株分为12组。约38个分离株表现出独特的模式。模式A发生在20％的分离物中。模式B，E和G的分离株均为细胞毒素阴性。其余组是细胞毒素阳性的。研究了从两个粪便标本中获得的多种分离株，以检查单个标本中REA分布的变化。在这些样品中，未发现REA分布的变化。通过检查单个分离株的连续体外传代培养和在两个月内从两个长期携带者分离的菌株的连续培养，研究了艰难梭菌的稳定性。无论是在体内还是体外，REA模式都随时间稳定。因为在53％的分离物中观察到质粒DNA，所以不能单独使用质粒图谱来研究艰难梭菌的传播。但是，在某些情况下，它们对于解释REA模式是必需的。

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