Early detection of influenza virus by using a fluorometric assay of infected tissue culture.

C T Pachucki; C Creticos

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Early detection of influenza virus by using a fluorometric assay of infected tissue culture.

机译：通过使用荧光检测受感染的组织培养物来早期检测流感病毒。

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A fluorometric substrate, 4-methylumbelliferyl-alpha-ketoside of N-acetylneuramide, was used directly on clinical specimens and infected tissue culture 24 h after inoculation for the detection of influenza viral neuraminidase. Viral neuraminidase was detected in infected tissue culture but not in clinical specimens. The sensitivity of the assay on tissue culture was 92%, and the specificity was 96%.

机译：N-乙酰神经酰胺的荧光底物，即4-甲基伞形酮-α-酮苷，直接用于临床标本和接种后24小时的感染组织培养物中，用于检测流感病毒神经氨酸酶。在感染的组织培养物中检测到病毒神经氨酸酶，但在临床标本中未检测到。该测定法对组织培养的敏感性为92％，特异性为96％。

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《Journal of Clinical Microbiology》 |1988年第12期|共3页
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C T Pachucki; C Creticos;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Early detection of influenza virus by using a fluorometric assay of infected tissue culture. [J] . C T Pachucki, C Creticos Journal of Clinical Microbiology . 1988,第12期

机译：通过使用荧光检测受感染的组织培养物来早期检测流感病毒。
2. Airborne influenza virus detection with four aerosol samplers using molecular and infectivity assays: considerations for a new infectious virus aerosol sampler [J] . P. Fabian, J. J. McDevitt, E. A. Houseman, Indoor Air . 2009,第5期

机译：使用四个气溶胶采样器使用分子和传染性测定法检测机载流感病毒：考虑使用新型传染性病毒气溶胶采样器
3. Use of Recombinant Nucleoproteins in Enzyme-Linked Immunosorbent Assays for Detection of Virus-Specific Immunoglobulin A (IgA) and IgG Antibodies in Influenza Virus A- or B-Infected Patients [J] . J. T. M. Voeten, J. Groen, D. van Alphen, Journal of Clinical Microbiology . 1998,第12期

机译：重组核蛋白在酶联免疫吸附测定中用于检测甲型或乙型流感病毒感染者的病毒特异性免疫球蛋白A（IgA）和IgG抗体
4. Evaluation of Pfizer Flu DETECT antigen test kit for the detection of laboratory isolates and experimentally infected swine with influenza A virus [C] . Annie Duquette, Jonathan Ertl, Marie Gramer, Annual^Meeting of the American Association^of^Swine^Veterinarians. . 2013

机译：掺锰流感检测抗原试剂盒的评价检测实验室分离株，用流感病毒进行实验感染猪
5. Development and Application of a Simple Molecular Short RT-PCR Assay for Detection and Differentiation of Influenza A Viruses. [D] . Benrahoma, Ghada Mohamed. 2013

机译：一种简单的分子短RT-PCR检测和鉴别甲型流感病毒的方法的开发和应用。
6. Early detection of influenza virus by using a fluorometric assay of infected tissue culture. [O] . C T Pachucki, C Creticos 1988

机译：通过使用荧光检测受感染的组织培养物来早期检测流感病毒。
7. Use of recombinant nucleoproteins in enzyme-linked immunosorbent assays for detection of virus-specific immunoglobulin A (IgA) and IgG antibodies in influenza virus A- or B-infected patients [O] . Groen J. (Jan), Alphen D. van, Claas E.C.J. (Eric), 1998

机译：重组核蛋白在酶联免疫吸附测定中用于检测流感病毒A或B感染患者的病毒特异性免疫球蛋白A（IgA）和IgG抗体的用途

Early detection of influenza virus by using a fluorometric assay of infected tissue culture.

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