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首页> 外文期刊>Journal of Clinical Microbiology >Routine viral culture for pediatric respiratory specimens submitted for direct immunofluorescence testing.
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Routine viral culture for pediatric respiratory specimens submitted for direct immunofluorescence testing.

机译:儿科呼吸道标本的常规病毒培养已提交直接免疫荧光测试。

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From 1986 to 1987, 69 (25%) of 274 specimens from children with lower respiratory tract syndromes were positive for respiratory syncytial virus antigen by direct immunofluorescence assay (DFA). Comprehensive viral culture was performed on all 205 DFA-negative specimens, and 72 specimens yielded viruses; 5 specimens yielded multiple agents. Thus, 52% of specimens yielded a specific virus, supporting the routine use of viral culture. Isolates from the DFA-negative specimens included respiratory syncytial virus (n = 7), rhinovirus (n = 34), hemadsorbing viruses (n = 13), cytomegalovirus (n = 11), adenovirus (n = 8), enteroviruses (n = 3), and herpes simplex virus (n = 2). Although serologic confirmation is needed, cytomegalovirus may be an underappreciated cause of acute lower respiratory tract infection in normal children. Further studies must be conducted to document this possibility.
机译:从1986年到1987年,通过直接免疫荧光分析(DFA),来自下呼吸道综合征儿童的274份标本中有69份(25%)的呼吸道合胞病毒抗原呈阳性。在所有205个DFA阴性标本上进行了全面的病毒培养,有72个标本产生了病毒。 5个标本产生多种试剂。因此,有52%的标本产生了特定的病毒,支持病毒培养的常规使用。来自DFA阴性标本的分离株包括呼吸道合胞病毒(n = 7),鼻病毒(n = 34),吸血病毒(n = 13),巨细胞病毒(n = 11),腺病毒(n = 8),肠病毒(n = 3)和单纯疱疹病毒(n = 2)。尽管需要血清学确认,但是巨细胞病毒可能是正常儿童急性下呼吸道感染的原因。必须进行进一步研究以证明这种可能性。

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