Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments.

M Eyers; S Chapelle; G Van Camp; H Goossens; R De Wachter

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Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments.

机译：通过聚合酶链反应扩增23S rRNA基因片段来区分嗜热弯曲杆菌。

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By comparing nucleic acid sequences determined for one of the most variable areas of 23S rRNA genes of 23 Campylobacter strains, we were able to identify regions specific for thermophilic Campylobacter strains. Oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. One primer pair selectively detected all thermophilic Campylobacter species, while four other primer pairs allowed discrimination among the thermophilic species Campylobacter coli, Campylobacter jejuni subsp. jejuni, Campylobacter lari, and Campylobacter upsaliensis. All primer sets were tested successfully on a large number of clinical isolates.

机译：通过比较为23个弯曲杆菌菌株的23S rRNA基因变化最大的区域之一确定的核酸序列，我们能够鉴定特异于嗜热弯曲杆菌菌株的区域。合成了与这些独特区域相对应的寡核苷酸引物，并将其用于聚合酶链反应中。一个引物对选择性地检测了所有嗜热弯曲杆菌物种，而其他四个引物对则可以区分嗜热弯曲杆菌大肠杆菌，空肠弯曲菌亚种。空肠，弧形弯曲杆菌和upsaliensis弯曲杆菌。所有引物组均已在大量临床分离株上成功测试。

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《Journal of Clinical Microbiology》 |1993年第12期|共4页
作者
M Eyers; S Chapelle; G Van Camp; H Goossens; R De Wachter;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Comparison of 23S polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism techniques as typing systems for thermophilic campylobacters [J] . Moreno Y., Ferrus MA., Vanoostende A., FEMS Microbiology Letters . 2002,第1期

机译：比较23S聚合酶链反应限制片段长度多态性和扩增片段长度多态性作为嗜热弯曲杆菌分型系统的技术
2. Characterization of nitrogen-fixing Paenibacillus species by polymerase chain reaction-restriction fragment length polymorphism analysis of part of genes encoding 16S rRNA and 23S rRNA and by multilocus enzyme electrophoresis [J] . Coelho MRR., von der Weid I., Zahner V., FEMS Microbiology Letters . 2003,第2期

机译：通过对部分编码16S rRNA和23S rRNA的基因进行聚合酶链反应-限制性片段长度多态性分析和多位点酶电泳来表征固氮芽孢杆菌
3. Quantification of mutated alleles of 23S rRNA in macrolide-resistant Campylobacter by TaqMan real-time polymerase chain reaction. [J] . Hao H, Dai M, Wang Foodborne pathogens and disease . 2010,第1期

机译：通过TaqMan实时聚合酶链反应定量分析大环内酯类耐药弯曲杆菌中23S rRNA的突变等位基因。
4. APPLICATION OF SYBR GREEN REAL-TIME POLYMERASE CHAIN REACTION FOR SIMULTANEOUS DETECTION OF CAMPYLOBACTER JEJUNI, ESCHERICHIA COLI O157:H7 AND SALMONELLA SPP [C] . H.M. Nam, B.E. Gillespie, S.E. Murinda, Annual Meeting of the National Mastitis Council . 2004

机译：SYBR绿色实时聚合酶链反应在同时检测云静电杆菌，大肠杆菌O157：H7和沙门氏菌SPP的应用
5. Molecular typing of Spiroplasma species and lines using rep-polymerase chain reaction and transposome mutagenesis and selection of natural non-adherent mutants of Spiroplasma citri. [D] . Mutaqin, Kikin Hamzah. 2005

机译：使用rep-聚合酶链反应和转座体诱变以及天然的非粘附性柠檬螺旋体突变体对螺旋体物种和品系进行分子分型。
6. Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments. [O] . M Eyers, S Chapelle, G Van Camp, 1994

机译：通过聚合酶链反应扩增23S rRNA基因片段来区分嗜热弯曲杆菌。
7. Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments [O] . M Eyers, S Chapelle, G Van Camp, 1993

机译：通过聚合酶链反应扩增23S rRNA基因片段的嗜热弯曲杆菌物种歧视
8. Cryptic Species in the Anopheles (Nyssorhynchus) albitarsis (Diptera: Culicidae) Complex: Incongruence Between Random Amplified Polymorphic DNA- Polymerase Chain Reaction Identification and Analysis of Mitochondrial DNA COI Gene Sequences. [R] . Lehr, M. A., Kilpatrick, C. W., Wilkerson, R. C., 2005

机译：按蚊（Nyssorhynchus）albitarsis（双翅目：蚊科）复合体中的隐蔽物种：随机扩增多态DNa-聚合酶链反应鉴定和线粒体DNa COI基因序列分析之间的不一致。

Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments.

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