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首页> 外文期刊>Journal of Clinical Microbiology >Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.
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Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.

机译：通过磁珠提取和临床样本中肠道病毒RNA的聚合酶链反应检测快速诊断肠道病毒感染。

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摘要

We describe a rapid method for extraction and detection of enterovirus RNA in clinical samples. By using magnetic bead technology, enterovirus RNA was efficiently and rapidly extracted from cerebrospinal fluid, stool, saliva, blood, pericardial fluid, urine, and cryopreserved or formalin-fixed solid tissue. Enterovirus RNA was then detected by reverse transcription followed by polymerase chain reaction amplification with primers designed to allow detection of most enterovirus serotypes. For detection of enteroviruses in specimens from patients with acute enteroviral disease, the overall sensitivity of enzymatic RNA amplification was greater than that of cell culture isolation, especially in blood specimens and in stool specimens from patients with acute cardiac disease. Enterovirus RNA was also detected in cryopreserved and archival formalin-fixed myocardial tissue from patients with acute myocarditis and chronic dilated cardiomyopathy. The ability to study archival specimens is of particular value in conducting retrospective investigation. The RNA extraction procedure used was considerably faster than extraction methods using organic reagents, used less hazardous reagents, and was of similar sensitivity. This detection protocol may therefore be useful both for the diagnosis of enterovirus infection and in studying the pathogenesis of acute and chronic enterovirus-induced disease.
机译：我们描述了一种用于临床样品中肠病毒RNA提取和检测的快速方法。通过使用磁珠技术，可从脑脊液，粪便，唾液，血液，心包液，尿液以及冷冻保存或福尔马林固定的固体组织中高效，快速地提取肠病毒RNA。然后通过反转录检测肠道病毒RNA，然后用设计用于检测大多数肠道病毒血清型的引物进行聚合酶链反应扩增。为了检测急性肠病毒病患者标本中的肠病毒，酶促RNA扩增的总体敏感性要高于细胞培养分离法，特别是在血液样本和急性心脏病患者粪便标本中。还在急性心肌炎和慢性扩张型心肌病患者的冷冻保存和福尔马林固定档案的心肌组织中也检测到肠病毒RNA。研究档案标本的能力在进行回顾性研究中具有特殊价值。所用的RNA提取程序比使用有机试剂的提取方法快得多，使用的危害性试剂更少，并且具有相似的灵敏度。因此，该检测方案对于诊断肠病毒感染以及研究急性和慢性肠病毒诱导的疾病的发病机理均可能有用。

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