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首页> 外文期刊>Journal of Clinical Microbiology >Evidence of multiple taxa within commercially available reference strains of Corynebacterium xerosis.
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Evidence of multiple taxa within commercially available reference strains of Corynebacterium xerosis.

机译：干燥棒状杆菌市售参考菌株中多个类群的证据。

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Attempts to identify coryneform isolates resembling Corynebacterium xerosis can lead clinical microbiologists to identification schemes with conflicting descriptions which result in confusing C. xerosis with Corynebacterium striatum. For the present study we purchased all available American Type Culture Collection and National Collection of Type Cultures reference cultures of C. xerosis (n = 10) and C. striatum (n = 4) and analyzed them as follows: (i) analysis of biochemical reactions in conventional tests and in the Rapid CORYNE system, (ii) whole-cell fatty acid analysis by using the gas-liquid chromatography research software of Microbial ID, Inc., and (iii) analysis of DNA homology in dot blot hybridizations. Three C. xerosis strains were indistinguishable from the C. striatum strains in whole-cell fatty acid analyses and DNA hybridizations and shared very similar biochemical reactions. The remaining seven strains of C. xerosis clustered into five groups on the basis of fatty acid patterns, DNA hybridizations, and biochemical tests. No reference strain of C. striatum fit the species description in Bergey's Manual of Systematic Bacteriology. The type strains of both C. striatum and C. xerosis fit their respective descriptions by the Centers for Disease Control and Prevention. This study suggests that the 10 commercially available reference strains of C. xerosis represent six different taxa which should be assigned to new species.
机译：尝试鉴定类似于干性棒状杆菌的棒状分离物会导致临床微生物学家采用描述矛盾的鉴定方案，从而导致干性棒状杆菌与纹状体棒状杆菌混淆。在本研究中，我们购买了所有可用的美国典型培养物保藏中心和美国典型培养物保藏中心，以干x衣藻（n = 10）和纹状体梭状芽胞杆菌（n = 4）为参考培养物，并进行如下分析：（i）生化分析（ii）使用Microbial ID，Inc.的气液色谱研究软件进行全细胞脂肪酸分析，以及（iii）点印迹杂交中的DNA同源性分析。在全细胞脂肪酸分析和DNA杂交中，三种干衣藻与纹状体菌株没有区别，它们具有非常相似的生化反应。根据脂肪酸模式，DNA杂交和生化测试，其余七个干枯萎病菌株可分为五个组。纹状梭菌的参照菌株不符合伯吉的《系统细菌学手册》中的物种描述。纹状体梭状芽胞杆菌和干性梭状芽胞杆菌的类型菌株均符合疾病控制和预防中心的描述。这项研究表明，十个商业上可获得的干枯病参照菌株代表了六个不同的分类单元，应该将它们分配给新物种。

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