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首页> 外文期刊>Journal of Clinical Microbiology >CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species.
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CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species.

机译：CHROMagar Candida，一种新的差异隔离培养基，用于推定临床上重要的念珠菌物种。

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CHROMagar Candida is a novel, differential culture medium that is claimed to facilitate the isolation and presumptive identification of some clinically important yeast species. We evaluated the use of this medium with 726 yeast isolates, including 82 isolated directly on the medium from clinical material. After 2 days of incubation at 37 degrees C, 285 C. albicans isolates gave distinctive green colonies that were not seen with any of 441 other yeast isolates representing 21 different species. A total of 54 C. tropicalis isolates also developed distinctive dark blue-gray colonies with a halo of dark brownish purple in the surrounding agar. C. krusei isolates (n = 43) also formed highly characteristic rough, spreading colonies with pale pink centers and a white edge that was otherwise encountered only rarely with isolates of C. norvegensis. Trichosporon spp. (n = 34) formed small, pale colonies that became larger and characteristically rough with prolonged incubation. Most of the other 310 yeasts studied formed colonies with a color that ranged from white to pink to purple with a brownish tint. The only exceptions were found among isolates identified as Geotrichum sp. or Pichia sp., some of which formed colonies with a gray to blue color and which in two instances formed a green pigment or a dark halo in the agar. The specificity and sensitivity of the new medium for the presumptive identification of C. albicans, C. krusei, and C. tropicalis exceeded 99% for all three species. A blinded reading test involving four personnel and 57 yeast isolates representing nine clinically important species confirmed that colonial appearance after 48 h of incubation on CHROMagar Candida afforded the correct presumptive recognition of C. albicans, C. tropicalis, C, krusei, and Trichosporon spp. None of nine bacterial isolates grew on CHROMagar Candida within 72 h, and bacteria (Escherichia coli) grew from only 4 of 104 vaginal, 100 oral, and 99 anorectal swabs. The new medium supported the growth of 19 of 23 dermatophyte fungi tested and 41 of 43 other molds representing a broad range of fungal pathogens and contaminants. In parallel cultures of 348 clinical specimens set up on Sabourand agar and CHROMagar Candida, both media grew yeasts in the same 78 instances. CHROMagar Candida is recommended as a useful isolation medium capable of the presumptive identification of the yeast species most commonly isolated from clinical material and facilitating recognition of mixed yeast cultures.
机译：CHROMagar Candida是一种新型的差异培养基，据称可促进某些临床上重要的酵母菌种的分离和推定鉴定。我们评估了该培养基与726株酵母菌分离物的使用，其中82株是从临床材料中直接从培养基中分离出来的。在37摄氏度下温育2天后，有285摄氏度的白色念珠菌分离物产生了独特的绿色菌落，而代表21个不同物种的441个其他酵母菌却没有出现。总共54个热带假丝酵母菌分离物还形成了独特的深蓝灰色菌落，在周围的琼脂中有暗褐色紫色的光晕。 C. krusei分离株（n = 43）也形成了特征鲜明的粗糙，散布的菌落，中心带有淡粉红色的中心和白色边缘，否则在C. norvegensis分离株中很少遇到。毛孢属（n = 34）形成小而浅的菌落，随着时间的延长，菌落变大且特征性地变得粗糙。研究的其他310种酵母菌中，大多数形成的菌落颜色从白色到粉红色再到紫色，带有淡褐色。在鉴定为Geotrichum sp。的分离株中发现了唯一的例外。或毕赤酵母属（Pichia sp。），其中一些形成了具有灰色至蓝色的菌落，并且在两种情况下，它们在琼脂中形成了绿色色素或暗色晕。对于这三个物种，用于推定鉴定白色念珠菌，克鲁斯念珠菌和热带念珠菌的新培养基的特异性和敏感性都超过了99％。对涉及9个临床上重要的物种的4名人员和57个酵母菌株进行的盲法阅读测试证实，在CHROMagar念珠菌上孵育48小时后，菌落的出现可以正确地推定白念珠菌，热带念珠菌，C，krusei和Trichosporon spp。 9个细菌分离株在72小时内没有在CHROMagar念珠菌上生长，细菌（大肠杆菌）仅从104个阴道拭子，100个口腔拭子和99个肛门直肠拭子中生长。新培养基支持了23种经测试的皮肤真菌的生长，以及43种其他霉菌中的41种的生长，它们代表了广泛的真菌病原体和污染物。在Sabourand琼脂和CHROMagar念珠菌上建立的348个临床标本的平行培养中，两种培养基均在相同的78个实例中生长了酵母。推荐使用CHROMagar Candida作为有用的分离培养基，能够推定鉴定最常从临床材料中分离出的酵母菌种，并有助于识别混合酵母培养物。

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