...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Typing of Candida krusei clinical isolates by restriction endonuclease analysis and hybridization with CkF1,2 DNA probe.
【24h】

Typing of Candida krusei clinical isolates by restriction endonuclease analysis and hybridization with CkF1,2 DNA probe.

机译：通过限制性核酸内切酶分析并与CkF1,2 DNA探针杂交来对克鲁斯假丝酵母临床分离株进行分型。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The use of restriction endonuclease analysis and Southern hybridization with our new CkF1,2 DNA probe, cold labeled with peroxidase, for the typing of Candida krusei isolates has been investigated. Fifty-five clinical samples isolated from forty-five patients hospitalized in eight centers, one environmental strain, and two reference strains were evaluated. Patterns were analyzed by a computer-assisted method and compared by numerical analysis. Clearer and less ambiguous patterns were obtained by restriction with endonuclease HinfI. It generated 9 to 14 (average, 11) well-separated fragments in the range of 6.5 to 2.0 kb. Both their numbers and sizes varied greatly among the strains studied. The CkF1,2 probe hybridized with one to seven fragments of HinfI patterns. A total of 48 distinct types were distinguished among the 58 strains studied. HinfI and CkF1,2 patterns showed similarities of less than 83 and 75% for unrelated strains and more than 91 and 100% for related strains, respectively. The methods showed 100% typeability, 98% reproducibility, and a discriminatory power of 1. C. krusei isolates from each patient were distinct, whether from one hospital or from different hospitals. Multiple isolates from the same patient were identical, both over time and at different anatomic sites. An endogenous origin is suggested for the colonizing and infecting isolates among the 45 patients. The CkF1,2 probe enhanced discrimination of the strains and provided a definitive comparison for strain identity. Genetic linkages between isolates were assessed at the subspecies level, and 12 clusters were delineated. A typing scheme is proposed for epidemiological studies of C. krusei.
机译：已经研究了限制性核酸内切酶分析和与我们新的CkF1,2 DNA探针（用过氧化物酶冷标记）的Southern杂交技术对克鲁斯假丝酵母菌株的分型的研究。从在八个中心住院的四十五名患者中分离出的五十五份临床样品进行了评估，其中一种是环境菌株，另外两种是参考菌株。模式通过计算机辅助方法进行分析，并通过数值分析进行比较。通过核酸内切酶HinfI的限制获得了更清晰和较少歧义的模式。它产生了9到14个（平均11个）分离得很好的片段，范围为6.5到2.0 kb。在所研究的菌株中，它们的数量和大小都相差很大。 CkF1,2探针与一到七个HinfI模式片段杂交。在所研究的58个菌株中，共鉴定出48种不同类型。 HinfI和CkF1,2模式的相似性分别显示出不相关菌株的相似度小于83和75％，相关菌株的相似度分别大于91和100％。该方法显示出100％的可打字性，98％的可重复性以及1的辨别力。无论是来自一家医院还是来自不同医院，每位患者的克鲁氏梭菌分离株都是不同的。随时间推移以及在不同解剖部位，来自同一患者的多个分离株是相同的。建议在45例患者中定殖和感染分离株为内源性。 CkF1,2探针增强了对菌株的区分，并提供了菌株鉴定的明确比较。在亚种水平上评估了分离株之间的遗传联系，并划定了12个簇。提出了一种用于克鲁氏梭菌流行病学研究的分类方案。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号