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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of a p21e-spiked western blot (immunoblot) in confirming human T-cell lymphotropic virus type I or II infection in volunteer blood donors. The Retrovirus Epidemiology Donor Study Group.
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Evaluation of a p21e-spiked western blot (immunoblot) in confirming human T-cell lymphotropic virus type I or II infection in volunteer blood donors. The Retrovirus Epidemiology Donor Study Group.

机译：评估p21e掺入的Western印迹（免疫印迹）以确认自愿献血者中的人T细胞I型或II型T细胞淋巴病毒感染。逆转录病毒流行病学捐助者研究组。

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Current algorithms for the serologic confirmation of human T-cell lymphotropic virus type I and II (HTLV-I/II) antibody reactivity are complicated. We evaluated the performance of an HTLV-I Western blot (immunoblot) spiked with recombinant p21e protein (p21e WB) as an alternative to current confirmatory methods. These methods include the HTLV-I viral lysate Western blot and either a radioimmunoprecipitation assay or a p21e enzyme-linked immunosorbent assay. Five hundred fifty nine blood donations obtained from five U.S. blood centers and classified as HTLV-I/II seropositive (n = 149) or seroindeterminate (n = 410) by routine testing methods were further evaluated by PCR for proviral DNA and by the p21e WB. On the basis of serologic and PCR testing, 155 donations were classified as HTLV-I/II infected. The sensitivity of the p21e WB was 97.4%, slightly exceeding that of routine confirmatory testing. The specificity of the p21e WB was 97.5%, as determined by testing of 404 seroindeterminate samples that were negative in the PCR. The positive predictive value of the p21e WB was 94%. In contrast, the specificity and positive predictive value of routine confirmatory testing were both 100%. Follow-up sampling of presumptive p21e WB false-positive donors substantiated the absence of HTLV-I/II infection. Although the p21e WB used in this study has high sensitivity and may be useful as a confirmatory assay in epidemiologic research studies, it may not be ideal as a confirmatory test for the notification of blood donors.
机译：用于I型和II型人T细胞淋巴病毒（HTLV-I / II）抗体反应性的血清学确认的当前算法很复杂。我们评估了掺入重组p21e蛋白（p21e WB）作为当前验证方法的替代品的HTLV-1 Western印迹（免疫印迹）的性能。这些方法包括HTLV-1病毒裂解物Western印迹和放射免疫沉淀测定法或p21e酶联免疫吸附测定法。通过常规检测方法从美国五个血液中心获得的559份献血被分类为HTLV-I / II血清阳性（n = 149）或血清不明确（n = 410），通过PCR对原病毒DNA和p21e WB进行了进一步评估。根据血清学和PCR检测，有155例捐赠被归类为HTLV-I / II感染。 p21e WB的灵敏度为97.4％，略高于常规验证性测试。 p21e WB的特异性为97.5％，这是通过检测404阴性的PCR阴性样品确定的。 p21e WB的阳性预测值为94％。相比之下，常规验证试验的特异性和阳性预测值均为100％。对p21e WB假阳性供体的随访抽样证实了不存在HTLV-I / II感染。尽管本研究中使用的p21e WB具有很高的灵敏度，并且可能在流行病学研究中用作确认试验，但它可能不是理想的用于通知献血者的确认试验。

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