Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam.

F Kawamoto; H Miyake; O Kaneko; M Kimura; T D Nguyen; T D Nguyen; Q Liu; M Zhou; D D Le; S Kawai; S Isomura; Y Wataya

首页> 外文期刊>Journal of Clinical Microbiology >Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam.

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Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam.

机译：越南南部椭圆形疟原虫中18S rRNA基因（基于PCR的疟疾诊断目标）的序列变异。

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Field surveys of malaria were performed in southern Vietnam by using an acridine orange staining method for rapid diagnosis and a PCR-based, microtiter plate hybridization method for accurate diagnosis. A total of three patients of Plasmodium ovale infection were detected, but PCR-amplified DNA of the P. ovale isolates from two of the patients did not hybridize with the P. ovale-specific probe. Analysis of the target sequence in the 18S rRNA gene indicated that in the DNA of isolates from both patients three nucleotides in the probe region from the typical P. ovale sequence were different, with deletions of two nucleotides and the substitution of one nucleotide. These results may suggest that in addition to molecular biological methods, careful microscopic examination of stained thin blood films is still required in studies of the prevalence of different malaria species.

机译：在越南南部，通过使用orange啶橙染色法进行快速诊断，并使用基于PCR的微量滴定板杂交法进行准确诊断，对疟疾进行了现场调查。总共检测到三例卵圆形疟原虫感染的患者，但是来自两名患者的卵圆形疟原虫分离株的PCR扩增DNA没有与卵圆形疟原虫特异性探针杂交。对18S rRNA基因中靶序列的分析表明，在这两名患者的分离株的DNA中，典型的卵圆形疟原虫序列在探针区域中的三个核苷酸是不同的，缺失了两个核苷酸并替换了一个核苷酸。这些结果可能表明，除了分子生物学方法以外，在研究不同疟疾种类的流行时，仍需要对染色的薄血膜进行仔细的显微镜检查。

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《Journal of Clinical Microbiology》 |1996年第9期|共3页
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F Kawamoto; H Miyake; O Kaneko; M Kimura; T D Nguyen; T D Nguyen; Q Liu; M Zhou; D D Le; S Kawai; S Isomura; Y Wataya;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Detection of Plasmodium ovale malaria parasites by species-specific 18S rRNA gene amplification. [J] . Tahar R, Basco LK Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines . 1997,第6期

机译：通过物种特异性18S rRNA基因扩增检测椭圆形疟原虫疟原虫。
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3. DNA sequence variation and determination of the putative PvCSP gene as potential vaccine target for Plasmodium vivax malaria in India [J] . Dash Manoswini Canadian Journal of Biotechnology . 2017,第SpecialaIssue期

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4. Molecular Phylogeny of Protura Inferred from 18S rRNA Gene Sequences [C] . Yun Bu, Yan Gao, Yunxia Luan, 第二届生物计算：理论及应用国际会议（The Second International Confere . 2007

机译：从18S rRNA基因序列推断Protura的分子系统发育。

5. Phylogenetic relationships of entoprocts and rotifers determined by nuclear 18s rRNA gene sequences. [D] . Mackey, Laura Yvonne. 1994

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6. Sequence variation in the 18S rRNA gene a target for PCR-based malaria diagnosis in Plasmodium ovale from southern Vietnam. [O] . F Kawamoto, H Miyake, O Kaneko, 1996

机译：越南南部椭圆形疟原虫的18S rRNA基因（基于PCR的疟疾诊断目标）的序列变异。

7. Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam [O] . F Kawamoto, H Miyake, O Kaneko, 1996

机译：18S rRNA基因的序列变异，PCR基疟疾诊断的靶标，来自越南南部的疟原虫卵巢

8. Comparison of the Nucleotide Sequence of Soybean 18S rRNA with the Sequences of Other Small-Subunit rRNAs [R] . Eckenrode, V. K., Arnold, J., Meagher, R. B. 1985

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3. Nucleotide sequences from RESA antigen of Plasmodium falciparum - used as probes and for prodn. of polypeptide(s) for use in vaccines and diagnosis of malaria [P] . 外国专利： PT84752A . 1987-05-01

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Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam.

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