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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of ligase chain reaction and culture for detection of Neisseria gonorrhoeae in genital and extragenital specimens.
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Comparison of ligase chain reaction and culture for detection of Neisseria gonorrhoeae in genital and extragenital specimens.

机译：连接酶链反应和培养物在生殖器和生殖器外标本中检测淋病奈瑟菌的比较。

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摘要

In addition to the urogenital tract, Neisseria gonorrhoeae infects extragenital sites such as the pharynx and anorectal canal. Culture and a ligase chain reaction (LCR)-based assay were compared for their performance for the diagnosis of N. gonorrhoeae infection with specimens from various urogenital and extragenital sites of 200 men and 125 women. The sensitivity and specificity of the LCR assay with male urethral swabs were both 100%, compared to values of 95.9 and 100%, respectively, for culture of urethral swabs or 98.0 and 100%, respectively, for LCR with first-void urine (FVU). For women, LCR with FVU showed the highest sensitivity (94.7%), and culture of urethral samples showed the lowest sensitivity (63.2%) (P < 0.05). In a selected subgroup of 47 men and 22 women at increased risk, the rates of pharyngeal infection were 15 and 18%, respectively, and those of anorectal infection were 13 and 45%, respectively. The sensitivity of LCR was greater than that of culture for both pharyngeal and anorectal specimens. Thus, the overall performance of LCR testing with swabs or FVU was better than that of culture for the diagnosis of genital or extragenital gonorrhea.
机译：除泌尿生殖道外，淋病奈瑟氏球菌还感染生殖器外部位，例如咽部和肛门直肠。用来自200名男性和125名女性的各种泌尿生殖器和生殖器外部位的标本，比较了基于培养物和基于连接酶链反应（LCR）的测定法在诊断淋病奈瑟氏球菌中的性能。雄性尿道拭子LCR测定的灵敏度和特异性均为100％，而尿道拭子培养的灵敏度分别为95.9和100％，初生尿液的LCR测定的敏感性和特异性分别为98.0和100％（FVU） ）。对于女性，带有FVU的LCR显示最高的敏感性（94.7％），而尿道标本的培养显示最低的敏感性（63.2％）（P <0.05）。在选定的47个风险较高的男性和22个女性的亚组中，咽部感染的发生率分别为15％和18％，肛门直肠感染的发生率分别为13％和45％。对于咽部和肛门直肠标本，LCR的敏感性均高于培养物。因此，用棉签或FVU进行LCR测试的总体性能要优于用于诊断生殖器或生殖器淋病的培养。

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