Two-Color Hybridization Assay for Simultaneous Detection of Bordetella bronchiseptica and Toxigenic Pasteurella multocida from Swine

Karen B. Register; Ruby M. Lee; Cindy Thomson

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Two-Color Hybridization Assay for Simultaneous Detection of Bordetella bronchiseptica and Toxigenic Pasteurella multocida from Swine

机译：同时检测猪支气管败血波氏杆菌和产多杀性巴斯德氏菌的双色杂交法

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Bordetella bronchiseptica and toxigenicPasteurella multocida are the etiologic agents of swine atrophic rhinitis. Methods currently used for their identification are time-consuming and suffer from a lack of sensitivity. We describe a colony lift-hybridization assay for detection of B. bronchiseptica and toxigenic P. multocida that can be performed with a single colony lift derived from a primary isolation plate without the need for pure subcultures of suspect bacteria. Membranes are hybridized simultaneously to probes derived from the B. bronchiseptica alcA gene and the P. multocida toxA gene. A multicolor development procedure permits sequential detection of bound probes. The assay was tested with 84 primary isolation plates generated from nasal swabs from swine with clinical signs of atrophic rhinitis. Comparison of the results from the colony lift-hybridization assay with those from conventional testing, based on a combination of colony morphology, biochemical reactions, mouse lethality, and enzyme-linked immunosorbent assay, indicated that the colony lift assay has superior sensitivity and comparable specificity. This technique has wide application for diagnostic and experimental studies.

机译：支气管败血博德特氏菌和产毒多杀性巴氏杆菌是猪萎缩性鼻炎的病原体。当前用于其识别的方法耗时且缺乏灵敏度。我们描述了用于检测 B的菌落杂交杂交测定法。支气管败血病和产毒 P。可以使用源自主要隔离板的单个菌落提升菌进行多菌种，而无需对可疑细菌进行纯继代培养。膜同时与衍生自 B的探针杂交。支气管败血病菌alcA 基因和 P。多杀性toxA 基因。多色显影程序允许顺序检测结合的探针。用84份从猪鼻拭子产生的具有萎缩性鼻炎临床体征的初级隔离板进行了检测。基于菌落形态，生化反应，小鼠致死率和酶联免疫吸附法的组合，将菌落提升杂交试验与常规测试的结果进行比较，表明菌落提升试验具有更高的灵敏度和相当的特异性。该技术在诊断和实验研究中具有广泛的应用。 展开▼

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《Journal of Clinical Microbiology》 |1998年第11期|共5页

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Karen B. Register; Ruby M. Lee; Cindy Thomson;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. Effect of Bordetella bronchiseptica and Serotype D Pasteurella multocida Bacterin-Toxoid on the Occurrence of Atrophic Rhinitis after Experimental Infection with B. bronchiseptica and Toxigenic Type A P. multocida [J] . Atsuyoshi TANEDA, Munenori OKADA, Shizuo SATO, The Journal of Veterinary Medical Science . 1997,第1期

机译：支气管败血波氏杆菌和血清型D多杀性巴斯德氏菌细菌类毒素对实验性支气管败血杆菌和产毒性多杀性伯氏杆菌感染后萎缩性鼻炎发生的影响

2. Serum haptoglobin concentration in growing swine after intranasal challenge with Bordetella bronchiseptica and toxigenic Pasteurella multocida type D. [J] . L Unverzagt, D P Bane, T R Shryock, Canadian Journal of Veterinary Research . 1996,第3期

机译：经支气管败血性博德特氏菌和D型多杀性巴斯德氏菌鼻内攻击后生长猪的血清触珠蛋白浓度。

3. Expression of the dermonecrotic toxin by Bordetella bronchiseptica is not necessary for predisposing to infection with toxigenic Pasteurella multocida [J] . Brockmeier SL, Register KB Veterinary Microbiology . 2007,第3a4期

机译：支气管败血性博德特氏菌表达皮肤坏死毒素并非易感产多杀性巴斯德氏菌感染

4. Protective efficacy of maternally derived passive immunity against virulent challenge by Bordetella bronchiseptica followed by toxigenic pasteurella multocida in suckling piglets [C] . Martin D. Ficken, Peggy Anne Hawkins Annual Meeting of the American Association of Swine Practitioners . 1999

机译：母标源性免疫对令人生畏的Bronchiseptica对毒性挑战的保护效果，然后在哺乳仔猪中进行毒性骨髓蛋白

5. Detection of Cystic Fibrosis Transmembrane Conductance Regulator DeltaF508 Gene Mutation Using a Paper-Based Nucleic Acid Hybridization Assay and a Smartphone Camera [D] . Malhotra, Karan. 2018

机译：纸质核酸杂交检测和智能手机相机检测囊性纤维化跨膜电导调节剂DeltaF508基因突变

6. Two-Color Hybridization Assay for Simultaneous Detection of Bordetella bronchiseptica and Toxigenic Pasteurella multocida from Swine [O] . Karen B. Register, Ruby M. Lee, Cindy Thomson 1998

机译：同时检测猪支气管败血波氏杆菌和产多杀性巴斯德氏菌的双色杂交法

7. Two-Color Hybridization Assay for Simultaneous Detection of Bordetella bronchiseptica and Toxigenic Pasteurella multocida from Swine [O] . Karen B. Register, Ruby M. Lee, Cindy Thomson 1998

机译：用于同时检测猪Bordetella Bronchiseptica和Toxigenic Pasteurella Multocad的双色杂交测定

1. 产毒素多杀巴氏菌和支气管败血波氏菌引起猪萎缩性鼻炎的比较... [J] . 褚桂芳 ,杨留战 . 中国畜禽传染病 . 1989,第005期

2. 支气管败血波氏杆菌、多杀性巴氏杆菌和副猪嗜血杆菌同时检测的PCR方法的建立 [J] . 邸海波 ,楚常欢 ,宋苍浩 . 畜禽业 . 2010,第005期

3. 用PCR检测猪产毒性多杀性巴氏标菌 [J] . . 中国动物保健 . 1999,第008期

4. 用支气管败血波氏杆菌和D型产毒多杀性巴氏杆菌鼻内攻毒后生长猪的血清结合 [J] . 马森 . 牧业译丛 . 1997,第003期

5. 产毒素多杀巴氏杆菌和支气管败血波氏杆菌在猪萎缩性... [J] . 杨留战 ,苑士祥 . 中国兽医科技 . 1992,第007期

6. 16S rRNA序列分析在多杀巴斯德氏菌鉴定中的应用 [C] . 高正琴 ,张强 ,邢进 . 2009年第七届中国北方实验动物科技年会暨第四届北京实验动物科学国际论坛 . 2009

7. 我国部分地区鸡群沙门氏菌感染的血清流行病学调查和抑制性差减杂交法筛选鸡白痢沙门氏菌特异性核酸序列 [A] . 徐耀辉 . 2005

1. 一种猪萎缩性鼻炎D型产毒多杀巴斯德氏菌疫苗株及其应用 [P] . 中国专利： CN107338208B . 2020.07.28

2. 一种猪萎缩性鼻炎D型产毒多杀巴斯德氏菌疫苗株及其应用 [P] . 中国专利： CN107338208A . 2017-11-10

3. Bivalent vaccine effective against atrophic rhinitis in pigs - contains dead Pasteurella multocida and Bordetella bronchiseptica as antigens (OE 15.10.78) [P] . 外国专利： DE2816942A1 . 1978-11-16

机译：对猪萎缩性鼻炎有效的二价疫苗-包含死亡的多杀巴斯德氏菌和支气管败血性博德特氏菌作为抗原（OE 15.10.78）

4. live attenuated bacterium of pasteurella multocida, live attenuated vaccine for the protection of animals or humans against infection by pasteurella multocida or its pathogenic effects, use of a live attenuated bacterium, method for the preparation of a vaccine, and diagnostic testing for the detection of dna or rna associated with pasteurella multocida [P] . 外国专利： BRPI0519381A2 . 2009-01-20

机译：多杀性巴氏杆菌的减毒活菌，用于保护动物或人类免受多杀性巴氏杆菌的感染或其致病作用的减毒活疫苗，减毒活菌的用途，疫苗的制备方法以及用于检测dna的诊断测试或与多杀性巴氏杆菌相关的RNA

5. MULTIPLEX REAL-TIME FLUORESCENCE PCR DETECTION PRIMER COMPOSITION AND DETECTION METHOD FOR IDENTIFYING STREPTOCOCCUS SUIS AND SWINE PASTEURELLA MULTOCIDA [P] . 外国专利： NL2024510A . 2020-07-07

机译：多重实时荧光PCR检测底漆组成及检测猪链球菌和猪多杀性巴斯德氏菌的方法

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Two-Color Hybridization Assay for Simultaneous Detection of Bordetella bronchiseptica and Toxigenic Pasteurella multocida from Swine

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